| Project/Area Number |
15591073
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
膠原病・アレルギー・感染症内科学
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| Research Institution | Fukuoka University |
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Principal Investigator |
TAKATA Tohru Fukuoka University, School of Medicine, Assistant Professor, 医学部, 講師 (90268996)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUMIYA Junji Fukuoka University, School of Medicine, Associate Professor, 医学部, 助教授 (70206556)
FUJIMOTO Shuji Kyushu University, School of Medicine, Associate Professor, 医学部, 助教授 (30199369)
KATSUTA Hitoshi Kyushu University, School of Medicine, Instructor, 医学部, 助手 (50333240)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Campylobacter jejuni / Helicobacter pylori / Cj0208 / Cj0634 / Cj0722c / natural transformation / methylase / DNAメチル転移酵素 / 病原因子 / DNA microarray |
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| Research Abstract |
I.Identification of the genes responsible for DNA uptake in Campylobacter jejuni-The role of a dprA ortholog(Cj0634) in Campylobacter jejuni was assessed. Strain 11168 was naturally competent for transformation by chromosomal DNA, with efficiency decreasing 100-fold in a Cj0634::aphA mutant, whereas strain 480 was not naturally competent. Strain 480 but not 11168 could be electro-transformed by shuttle plasmid pRY111, an effect completely abolished by Cj0634 interruption. Complementation of the Cj0634 mutation in strain 480 in trans with vectors containing the dprA homolog from C.jejuni, Helicobacter pylori, or Haemophilus influenzae, completely (for Cj0634) or partially (H.pylori >H.influenzae) restored electro-transformation. Thus, C.jejuni possesses dprA that functionally most closely resembles that of H.pylori and is involved in DNA transformation.
II.Identification of the function of putative DNA methylase (Cj0208) in C.jejuni : The protein expression profile of an isogenic Cj0208 mutant then was compared with that of the wild-type. 10 proteins including flagellar proteins were expressed more abundantly in the wild-type. Using microarray analysis, the flagellin genes (flaA and flaB) were less transcribed in the mutant compared to the wild-type amongst other differences. The wild-type strain colonized the intestinal tracts with >10^9CFU in all 4 chicks infected, while the Cj0208 mutant colonized 2-3 logs less in 3 chicks and was not detectable in the fourth chick. These results suggest that methylase activity of Cj0208 affects expression at the transcriptional and protein level of at least several genes, and that this methylation has consequences for colonization.
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