Neural stem cell transplantation for childhood acute encephalomyelitis using the animal model
| Project/Area Number |
15591097
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Shinshu University School of Medicine |
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Principal Investigator |
ICHIKAWA Motoki Shinshu University, School of Medicine, Professor, 医学部, 教授 (60223088)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAYAMA Kozo Shinshu University, School of Medicine, Assistant Professor, 医学部, 講師 (70192680)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | neural stem cell transplantation / experimental autoimmune encephalomyelitis / 神経幹細胞 / 実験的自己免疫性脳脊髄 |
|---|
| Research Abstract |
We have studied the effect of neural stem cell transplantation for the treatment of experimental autoimmune encephalomyelitis (EAE). The neural stem cells were obtained form the fetal rat brain and were introduced enhanced green fluorescence protein (EGFP) gene. We transplanted these cells to the cerebral hemisphere of the Lewis rat with EAE induced by sensitization of guinea pig myelin basic protein. However, we could not find the difference of the clinical scores of encephalomyelitis between neural stem cell transplanted group and no treatment group. We performed the autopsy after encephalomyelitis recovery and made histopathology specimens of central nervous system and checked the distribution of EGFP positive cells with fluorescence microscope. However, the EGFP positive transplanted neural stem cells were not found at the central nervous system tissues of the rat. Next, we transplanted the EGFP positive neural stem cells to the cerebral hemisphere of NOD mice with EAE induced by sensitization of myelin oligodendrocyte glycoprotein peptide 35-55. These mice caused optic neuritis and small amount of the EGFP positive cells were observed at the optic nerve tissues. However, the difference was not noted in recovery of clinical manifestation between neural stem cell transplanted group and no treatment group. It is necessary to study about the survival of the transplanted neural stem cells for the treatment of neural stem cell transplantation in EAE.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] The Effects of close contact between mothers and their babies on the heart rate variability and feeling towards the infant2006
Author(s)
Kesami Sakaguchi, Masayoshi Oohira, Motoki Ichikawa, Noriko Ookubo, Shigeko Sakaguchi, Minoru Hongo, Makoto Kanai, Noriko Fukushima, Rie Kondo, Takaya Yanagihashi
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Journal Title
Japanese Journal of Maternal Health Vol 47, No 1
Pages: 190-196
NAID
Description
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[Journal Article] Cerebellar ataxia in pediatric patients with Langerhans cell histiocytosis2004
Author(s)
Imashuku S, Ishida S, Koike K, Ichikawa M, Minato T, Suzuki Y, Kobayashi R, Morimoto A, Japan LCH Study, Group
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Journal Title
Journal of Pediatric Hematology Oncology Vol 26, No 11
Pages: 735-739
Description
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[Book] 事例で学ぶ母性看護学2004
Author(s)
大井けい子, 玉熊和子, 新道幸恵, 大関信子, 益田早苗, 高橋佳子, 大久保功子, 市川元基, 鈴木泰子
Total Pages
152
Publisher
メヂカルフレンド社
Description
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