Early diagnosis of disseminated adenovirus infection associated with hematopoietic stem cell transplantation by quantitative PCR
| Project/Area Number |
15591123
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
MORIMOTO Akira Kyoto Prefectural University of Medicine, Pediatrics, Assistant Professor, 医学研究科, 講師 (30326227)
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| Co-Investigator(Kenkyū-buntansha) |
UEDA Ikuyo Kyoto Prefectural University of Medicine, Pediatrics, Assistant Professor, 医学研究科, 助手 (00381939)
今宿 晋作 京都市衛生公害研究所, 所長
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Adenovirus infection / Hematopoietic Stem cell transplantation / Polymerase chain reaction / Real-time PCR / Hemorrhagic cystitis / Pneumonitis / Hematopoietic Stem cell transplantation / Hematopietic Stem cell transplantation / Rcal-time PCR |
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| Research Abstract |
A 17-year-old male who had undergone bone marrow transplantation (BMT) died of multiple organ failure. We evaluated his serum and urine samples for adenovirus (AdV) genome using real-time PCR retrospectively. AdV DNA copy numbers, increasing at first in urine followed by in serum, were closely related with his clinical course.
A 12-year-old female developed life-threatening pneumonia with hemophagocytic syndrome. We could not pin down pathogen with antibody titer or culture. However, PCR assay of lung tissue was positive for AdV and real-time PCR analysis confirmed this diagnosis.
We evaluated serum and urine samples of 7 patients received BMT for AdV genome using real-time PCR in time course retrospectively. With this analysis 3 of 7 patients were confirmed to have disseminated AdV infection. In all of these 3 patients, AdV DNA was found to be positive in urine at one or two weeks after BMT and those were to be more than 10^5 copies/ml in serum at 4 to 6 weeks after BMT. 2 patients, who were found to have more than 10^3 copies/ml AdV in serum before BMT, developed disseminated AdV infection.
A 17-year-old female who was received BMT developed intractable diarrhea and spiky fever. Rapid AdV antigen detection test was positive in stool, however, PCR using conventional primers for AdV failed to detect AdV DNA because of 9 base substitutions in this AdV. She was confirmed to have AdV infection by PCR analysis using AdV type 7 specific primers.
Quantification of AdV copies is powerful method for early diagnosis of disseminated AdV infection. Patients who are positive for serum AdV copies before BMT and found to be positive for urine AdV copies after BMT are high risk of developing disseminated AdV infection. Using this technique we can detect AdV in biopcied specimens such as lung. However we have to pay attention for false negative results in PCR analysis because AdV has mutations frequently.
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Report
(4 results)
Research Products
(10 results)
