| Project/Area Number |
15591126
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Osaka City University |
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Principal Investigator |
MATSUOKA Osamu Osaka City University, Graduate School of Medicine, Lecturer, 大学院・医学研究科, 講師 (20117972)
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| Co-Investigator(Kenkyū-buntansha) |
YAMANO Tunekazu Osaka City University, Graduate School of Medicine, Professor, 大学院・医学研究科, 教授 (20093172)
HATTORI Hideji Osaka City University, Graduate School of Medicine, Lecturer, 大学院・医学研究科, 講師 (70244639)
OGURA Hisashi Osaka City University, Graduate School of Medicine, Professor, 大学院・医学研究科, 教授 (10115222)
AYATA Minoru Osaka City University, Graduate School of Medicine, Research, 大学院・医学研究科, 助手 (90222702)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2004: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2003: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | Subacute Sclerosing Panencephalitis / SSPE virus / measles virus / cell tropism / SLAM (CD150) / CD46 / SLAM(CD150) |
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| Research Abstract |
Our results obtained so far suggested a possibility of the 3rd receptor for SSPE strains different from CD150 (SLAM) and CD46. The purpose of this project is to further confirm existence of this novel receptor and to clone cDNA of this molecule.
To confirm its existence, virus entry assay was performed using VSV pseudotype system together with entry blocking by the specific monoclonal antibody against CD150 or CD46. SSPE strains were found to use CD150 but not CD46, in addition, a certain cell surface molecule except for the both ones. This novel molecule was expressed human neuroblastoma cells and human glioma cells. As another estimation on virus entry, syncytium formation by SSPE psedovirions prepared by treatment of the infected cells with cytochalasin D was monitored and the same results as VSV pseudotype system were also obtained.
Based on the above confirmation, cDNA cloning of the 3rd receptor was tried using ecotropic retroviral vector from cDNA library of Vero cells, which seemed to express the molecule most among our available cells examined. In addition to it, preparation of mouse monoclonal antibody blocking entry of SSPE strains into Vero cells was tried to catch the novel molecule. At present, we have not yet succeeded probably because of too little amount of its expression. These are now still in progress. As a different approach, some more sensitive and effective cloning methods are under consideration.
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