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Functional significance of L1 cell adhesion molecule in the brain development of L1-mutant mice

Research Project

Project/Area Number 15591160
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Embryonic/Neonatal medicine
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

ITOH Kyoko  Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Associate Professor, 医学研究科, 助教授 (80243301)

Co-Investigator(Kenkyū-buntansha) FUSHIKI Shinji  Kyoto Prefectural University of Medicine, Graduate School of Medical Science, Professor, 医学研究科, 教授 (80150572)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2003: ¥3,100,000 (Direct Cost: ¥3,100,000)
KeywordsL1CAM / brain development / mutant mice / immunohistochemistry / 脳神経系発達
Research Abstract

In order to elucidate the pathogenetic mechanisms of hydrocephalus due to L1CAM gene mutations, we evaluated brain development spatiotemporally of both L1-null mutant mice and L1-6D mice devoid of 6th immunoglobulin domain of L1. Genetic background of them was C57BL/6. We performed morphological analyses of fetal brains from embryonic day (E) 12.5 to 16.5. Genetic typing was done using amniotic membranes by PCR method. Backcrossing the L1-6D mice onto the C57BL/6 strain (B6-L1-6D) produced embryonic lethality. The yield of B6-L1-6D male(-/Y) was 15% of the litter at E12.5 and 6% at birth, being higher than expected (25%). The yield of B6-L1KO was 13% at E12.5, 16% at E16.5 and 5% at birth, which suggested the factors leading to lethality might act at late embryonic phase. The brains of B6-L1-6D showed normal development, including retina, retinotectal tract, telencephalon, diencephalons, midbrain, brainstem, spinal cord and dorsal root ganglia at E12.5. The brains of B6-L1KO showed decreased number of TUJ1-immunoreactive ganglion cells of retina, poor growth of retinotectal tract and hypoplastic postoptic recess at E12.5, which was alleviated at E16.5. Further analyses using other methods such as in vivo electroporation with siRNA might be beneficial to understand functional significance of L1 during brain development.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (8 results)

All 2004 Other

All Journal Article (6 results) Publications (2 results)

  • [Journal Article] Brain development in mice lacking L1-L1 homophilic adhesion.2004

    • Author(s)
      Itoh K, Cheng L, Kamei Y, Fushiki S, Kamiguchi H, Gutwein P, Stoeck A, Arnold B, Altevogt P, Lemmon V
    • Journal Title

      Journal of Cell Biology 165

      Pages: 145-154

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 中枢および末梢神経系発達における神経細胞接着分子(L1CAM)の意義2004

    • Author(s)
      伊東恭子
    • Journal Title

      京府医大誌 113(2)

      Pages: 69-82

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] X連鎖性遺伝性水頭症のモデル動物2004

    • Author(s)
      伊東恭子, 上口裕之
    • Journal Title

      細胞工学 23(12)

      Pages: 1414-1417

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Brain development in mice lacking L1-L1 homophilic adhesion.2004

    • Author(s)
      Itoh K, Cheng L, Kamei Y, Fushiki S, Kamiguchi H, Gutwein P, Stoeck A, Arnold B, Altevogt P, Lemmon V
    • Journal Title

      Journal of Cell Biology 165(1)

      Pages: 145-154

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Functional significance of L1 cell adhesion molecule in the development of central and peripheral nervous systems.2004

    • Author(s)
      Kyoko Itoh
    • Journal Title

      J.Kyoto Pref.Univ.Med. 113(2)

      Pages: 69-82

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Model animals for X-linked hydrocephalus.2004

    • Author(s)
      Kyoko Itoh, Hiroyuki Kamiguchi
    • Journal Title

      CELL TECHNOLOGY 23(12)

      Pages: 1414-1417

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Kyoko Itoh: "Brain development in mice lacking L1-L1 homophilic adhesion"Journal of Cell Biology. 165(1). 1-11 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 伊東 恭子: "中枢および末梢神経系発達における神経細胞接着分子(L1CAM)の意義"京都府立医科大学雑誌. 113(2). 69-82 (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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