| Project/Area Number |
15591202
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Kurume University, School of Medicine |
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Principal Investigator |
KUSUHARA Masahiro Kurume University, School of Medicine, assistant professor, 医学部, 講師 (40195441)
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| Co-Investigator(Kenkyū-buntansha) |
HASHIMOTO Takashi Kurume University, School of Medicine, professor, 医学部, 教授 (20129597)
YASUMOTO Shinichiro Kurume University, School of Medicine, associate professor, 医学部, 助教授 (10220162)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2003: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | dendritic cell / Langerhans cell / substance P / TNF-α / CD80 / LPS / MHC class II |
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| Research Abstract |
Immature dendritic cells, which cultured from bone marrow of BALB/c mouse with mouse recombinant GM-CSF, were stimulated with TNF-α for 24 hours. The expression of CD80 on cell surface was markedly inhibited by some concentration of substance P. Lipopolysaccharide and IFN-γ didn't affect to maturation of cells. The cytokine productions, IL-12p70,IFN-γ,IL-10 and IL-2, in supernatant of cell culture were not effected by addition of substance P. Mouse Langerhans cell sorted from mouse epidermis were stimulated with LPS,IFN-γ and TNF-α, but no markedly changes were observed in the expression of maturation marker and cytokine production. There is some possibility that dendritic cells except Langerhans cell are inhibited in antigen presenting function in a concentration of substance P. In the examination of cell migration, the number of Langerhans cells, which migrated from FITC-applied skin with or without subcutaneously injection of substance P, in draining lymph node was counted by using flow cytometry, but there are no differences in numbers. The splenocytes of OVA-immunized BALB/c mouse and OVA-pulsed bone marrow derived dendritic cell or sorted Langerhans cells were cultured with suhstance P, and cell proliferation was checked with CFDA SE fluorescence dye. However the proliferations of CD4^+ T cells by both cells were not observed, CD8^+ T cells proliferation by cultured dendritic cells was promoted in some concentration of substance P. There is the possibility of effects on antigen specific cytotoxic T cell activity by dendritic cell, which stimulated by substance P.
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