Expression of candidate susceptibility gene in the cingulated cortex of brains from patients with schizophrenia : a postmortem study
| Project/Area Number |
15591224
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Psychiatric science
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| Research Institution | Kobe University |
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Principal Investigator |
HASHIMOTO Takeshi Kobe University, Hospital, Lecturer, 医学部附属病院, 講師 (60294229)
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| Co-Investigator(Kenkyū-buntansha) |
MAEDA Kiyoshi Kobe University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (80116251)
KAWAMATA Toshio Kobe University, School of Medicine, Professor, 医学部, 教授 (70214690)
SHIRAKAWA Osamu Kobe University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (40243307)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2003: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | schizophrenia / postmortem brain / mRNA / cDNA array / PCP / SAPAP / RGS |
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| Research Abstract |
In our Scientific Research (C)(2) project from 2003 to 2004, we investigated gene expression in the cingulate and prefrontal cortices of postmortem brains of schizophrenia, in order to identify novel candidate genes for schizophrenia.
1)A cDNA array was used to screen pools of samples from the cingulate or prefrontal cortex of patients with schizophrenia. Of the examined genes, 33 mRNA were increased and 22 were decreased. Quantitative reverse transcriptase (RT)-PCR, Western blot, or in situ hybridization analyses were done to confirm the results. We screened for genetic variations in the possible candidate genes and studied the association between schizophrenia and genetic polymorphisms.
2)Using differential display, we identified a gene regulated by the delayed action of PCP in rat nucleus accumbens (NAcs). The cDNA clone obtained was identical to rat synapse-associated protein 90/postsynaptic density-95-associated protein 1 (SAPAP1). Immunoquantification using an anti-SAPAP1 antibody indicated that immunoreactivity for SAPAP1 increased in the NAcs of postmorten brains from unmedicated patients with schizophrenia. We identified a single nucleotide polymorphism in the SAPAP1/DAP-1 gene (1618A/G). However, a case-control study did not reveal a significant association between this polymorphism and schizophrenia.
3)We found a novel missense polymorphism (Val38Met) in the Regulator of G-protein signaling 10 (RGS10) gene. A case-control study did not show a significant association between this polymorphism and schizophrenia.
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Report
(3 results)
Research Products
(10 results)
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[Journal Article] Association of 14-3-3 epsilon gene haplotype with completed suicide in Japanese.2005
Author(s)
Yanagi M, Shirakawa O, Kitamura N, Okamura K, Sakurai K, Nishiguchi N, Hashimoto T, Nushida H, Ueno Y, Kanbe D, Kawamura M, Araki K, Nawa H, Maeda K.
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Journal Title
J Hum Genet. 50(4)
Pages: 210-216
Description
「研究成果報告書概要(欧文)」より
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