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Free -radical generation by amyloid p protein and the protective effect by laminin against amyloid β-protein induced toxicity

Research Project

Project/Area Number 15591235
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Psychiatric science
Research InstitutionUniversity of Miyazaki (2004)
宮崎医科大学 (2003)

Principal Investigator

HAYASHI Yoshihito  University of Miyazaki, Faculty of Medicine, Dept of Psychiatry, assistant, 医学部, 助手 (20295223)

Co-Investigator(Kenkyū-buntansha) UEDA Yuto  University of Miyazaki, Faculty of Medicine, Dept of Psychiatry, assistant professor, 医学部, 助教授 (70244192)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordsβ amyloid / laminin / free radical / NMDA
Research Abstract

We investigated the inhibitory effect by laminin, which is one of the extracellular matrix components, against beta amyloid(Aβ) induced free radicals by electron paramagnetic resonance(EPR) using PC12 cells with two kinds of spin trap, 5,5-dimethyl-1-pyrroline-N-oxide ; DMPO, α-(4-pyridyl-1-oxide)-N-tert-butylnitrone ; POBN respectively. We investigated two kinds of experiment to clear the mechanism how laminin shows the protection against Aβ. At first, EPR measurement was performed to see whether or not laminin directly inhibit Aβ induced free radicals, Aβ was mixed with laminin and the solution was added to PC12 cells. The second, EPR measurement was also performed to see whether or not the cells which attached laminin get strengthened their defense function.
The free radical production was reduced when Aβ was mixed with laminin and the cell viability also increased compared with Aβ only added to the cells, though there was no difference in generating free radicals between cells which were attached with laminin for 24hrs before Aβ addition and cells which did not attach laminin for 24hrs. Therefore we concluded that laminin has neuroprotective ability and its protective effect against Aβ induced free radical generation is due to possibly direct reaction with Aβ.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (2 results)

All 2004

All Journal Article (2 results)

  • [Journal Article] EPR evidence of hydroxyl radical generation as an initiator of lipid paroxidation in amyloid β-protein-stimulated PC12 cells2004

    • Author(s)
      Yoshihito Hayashi, Yuto Ueda, Akira Nakajima, Yoshio Mitsuyama
    • Journal Title

      Brain Research 1025

      Pages: 29-34

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] EPR evidence of hydroxyl radical generation as an initiator of lipid peroxidation in amyloid β-protein-stimulated PC12 cells2004

    • Author(s)
      Yoshihito Hayashi, Yuto Ueda, Akira Nakajima, Yoshio Mistuyama
    • Journal Title

      Brain Research 1025

      Pages: 29-34

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary

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Published: 2003-04-01   Modified: 2016-04-21  

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