| Project/Area Number |
15591365
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | NIHON UNIVERSITY |
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Principal Investigator |
AMANO SADAO NIHON UNIVERSITY, SCHOOL OF MEDICINE, ASSOCIATE PROFESSOR, 医学部, 講師 (80159459)
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| Co-Investigator(Kenkyū-buntansha) |
AOKI NOBUHIKO NIHON UNIVERSITY, SCHOOL OF MEDICINE, ASSOCIATE PROFESSOR, 医学部, 講師 (70318393)
KASHIO MITUHIKO NIHON UNIVERSITY, SCHOOL OF MEDICINE, CLINICAL FELLOW, 医学部, 助手 (20343577)
ENOMOTO KATSUHISA NIHON UNIVERSITY, SCHOOL OF MEDICINE, CLINICAL FELLOW, 医学部, 助手 (60386026)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | VEGF-C / Lymphedema / Lymphangiogenesis / Gene transfer / post operative state of breast cancer / in vivo electroporation |
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| Research Abstract |
We have examined the relationship between the vascular endothelial growth factors (VEGE-family) and the character of cancer (Mori, Saitou, Kuboi, et al.). On the other hand, the patients who received axillary dissection and postoperative irradiation for the breast cancer produce the lymphedema of the suffered arm that are difficult to cure (Kitajima et al.). We conducted the experimental study of the gene transfer of VEGF-C (lymphatic duct growth factor) to determine the effect and safety of this therapy for lymphatic edema animal model.
Method : The pCAGGS-VEGF-C expression plasmid was used to assess the efficiency of gene transfer into muscle by electroporation in vivo. This vector was prepared by inserting a VEGF-C cDNA into the pCAGGS expression vector containing the CAG promoter. In vivo electroporation method was performed using the gene transfer equipment CUY21EDIT. The surgical lymphedema by the method of Kawashima was created in the leg of one side of the rat. The rat were injected with 50 μg each of closed circular plasmid DNA (pCAGGS-VEGF-C, control pCAGGS). Circumference measurement was performed to calculated percent difference and circumference reduction rate. The tibialis anterior muscles samples for sacrificed rat were fixed 10% formalin in PBS for 24hours. Serial cut sections were sliced for hematoxylin-eosin (H-E) and immunohistochemical staining to evaluate lymphatic vessel findings (vessel density and vessel area rate) histologically. Results : The percent difference and the circumference reduction rate respectively showed between VEGF group and control. However, a significant difference was not found between the treatment group and control group. Lymphatic vessels density and vessel area rate were also increased in treatment group however ; there was no significant difference between the two groups. Conclusion : In vivo electroporation gene transfer of VEGF-C cDNA was demonstrated likelihood of effective to the lymphedema.
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