| Project/Area Number |
15591384
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KARAKO Takashi The University of Tokyo, Faculty of Medicine, Assistant, 医学部附属病院, 助手 (00313213)
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| Co-Investigator(Kenkyū-buntansha) |
MAKUUCHI Masatoshi The University of Tokyo, Faculty of Medicine, Professor, 医学部附属病院, 教授 (60114641)
KOKUDO Norihiro The University of Tokyo, Faculty of Medicine, Associate Professor, 医学部附属病院, 助教授 (00205361)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2003: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Colorectal Cancer / live metastasis / Metastasis / Glycocojugates / Lectins / Sialic acid / Immunohistochemistry / Prognosis |
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| Research Abstract |
Sialoglycoconjugates is thought to play an important role in tumor metastasis and progression. This study involved histochemical analyses of human colorectal cancer and metastatic liver cancer tissues to detect tumor-associated sialoglycoconjugates and to clarify the relationship between their histochemical expression and the metastatic potential of the tumor.
Human colorectal cancer and metastatic tissues were subjected histochemical staining using sialic acid-binding lectins and anti-KL-6 mucin monoclonal antibody. Relationship between the staining characteristics and clinicopathological parameters were statistically analyzed. Aberrant expression of sialoglycoconjugates was detected in colorectal cancer tissues by means of lectin-histochemical staining. The high level staining was related to aggressive tumor behavior such as lymphatic metastasis. On the other hand, KL-6 mucin was detected in colorectal cancer tissues but not in normal tissues surrounding the tumors. The expression was significantly frequent not only in cases of lymph node metastasis but also in those of liver metastasis. In addition, KL-6 antigen was detected in metastatic liver cancer tissues with high frequency (53/56, 95%).
In conclusion, the present results indicate that tumor-associated sialoglycoconjugates appear in human colorectal cancer and metastatic liver tissues and suggest that the aberrant expression of sialoglycoconjugates, especially KL-6 mucin, is closely related to unfavorable behavior such as liver metastasis of colorectal cancer.
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