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Establishment of diagnostic system by immunohistochemistory using Tissue Microarray for human gastric cancer

Research Project

Project/Area Number 15591388
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionTokyo Medical and Dental University

Principal Investigator

YAMADA Hiroyuki (2004)  Tokyo Medical and Dental University, Faculty of Medicine, assistant, 医学部附属病院, 助手 (70313244)

山下 俊樹 (2003)  東京医科歯科大学, 医学部附属病院, 助手 (80334415)

Co-Investigator(Kenkyū-buntansha) UETAKE Hiroyuki  Tokyo Medical and Dental University, Faculty of Medicine, assistant, 医学部附属病院, 助手 (60311651)
OSANAI Takeyuki  Tokyo Medical and Dental University, Graduate School, assistant, 学院医歯学総合研究科, 助手 (50301164)
KOJIMA Kazuyuki  Tokyo Medical and Dental University, Graduate School, lecturer, 大学院・医歯学総合研究科, 講師 (60313243)
SUGIHARA Kenichi  Tokyo Medical and Dental University, Graduate School, professor, 大学院・医歯学総合研究科, 教授 (10171167)
山田 博之  東京医科歯科大学, 医学部附属病院, 助手 (70313244)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsTissue Microarray / immunity histology experiment / Donor block / Array block / Recipient block / cancer-related protein
Research Abstract

Forty-one gastric cancer specimens (20 were differentiated adenocarinoma and 21 undifferentiated) were analyzed in this study. Using Manual tissue arrayer, 2 grafts (2mm in diameter), which showed representative histological feature, were taken out as from each specimen ("donor block"). Forty-eight grafts were put per "recipient block". The graft was lined up in the donor block by histological type and tumor stage. In cutting the recipient block, successfully cut into 3 micro-meter thickness without the grafts in the recipient block being destroyed. It became clear that an array block well reflected the histological structure of donor blocks in tumor cell and gland density when it was compared with donor blocks. In immunohistochemistory, a positive correlation was observed in p-53, Ki-57 and COX-2 expression intensity between donor and microarray (p<0.0005, <0.0005 and <0.0001, respectively). Therefore, diagnostic system by immunohistochemistory using Tissue Microarray was supposed to be useful for human gastric cancer
We are now evaluating the efficacy of Tissue Microarray by analyzing the expression of other proteins, such as VGEF-C, thymidylate synthase, dihydropyrimidine dehydrogenase, thymidine phosphorylase and so on..

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (10 results)

All 2005 2004

All Journal Article (10 results)

  • [Journal Article] Thymidylate synthase and dihydropyrimidine dedydrogenase gene expression in breast cancer predicts 5-FU chemosensitivity by a histocultural drug sensitivity test2005

    • Author(s)
      柿本應貴
    • Journal Title

      CANCER LETTERS (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] JTE-522, a selective COX-2 inhibitor, inhibits growth of pulmonary metastases of colorectal cancer in rats2005

    • Author(s)
      小松宏寿
    • Journal Title

      BMC CANCER 5

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Thymidylate synthase and dihydropyrimidine dedydrogenase gene expression in breast cancer predicts 5-FU chemosensitivity by a histocultural drug sensitivity test2005

    • Author(s)
      Kakimoto M
    • Journal Title

      CANCER LETTERS (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] JTE-522, a selective COX-2 inhibitor, inhibits growth of pulmonary metastases of colorectal cancer in rats2005

    • Author(s)
      Kobayashi H
    • Journal Title

      BMC CANCER 5:Art.No.26 MAR 3

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A phase I/II study of tegafur-uracil (UFT) plus oral leucovorin (LV) and biweekly CPT-11 therapy for patients (pts) with metastatic colorectal cancer (CRC).2004

    • Author(s)
      植竹宏之
    • Journal Title

      JOURNAL OF CLINICAL ONCOLOGY 22

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] E-Selectin-Triggered Recruitment of Endothelial Progenitor Cells to Vascular Endothelium is Significantly Enhanced by Preexisting Plateles.2004

    • Author(s)
      N.Nakamura, Y.Nishiwaki, M.Yoshida, K.Shimokado
    • Journal Title

      Circulation J. Suppl. 68

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Identification of the critical serine residues responsible for phosphorylation of endothelial E-selectin.2004

    • Author(s)
      M.Nagai, N.Nitta, K.Shimokado, M.Yoshida
    • Journal Title

      Circulation J. Suppl. 68

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Sphinsosine l-phosphate induced monocyte-endothelial interaction involves PKC and RhoA dependent mechanism2004

    • Author(s)
      A.Kawakami, M.Nagai, A.Tanaka, K.Nakajima, K.Shimokado, M.Yoshida
    • Journal Title

      Circulation J. Suppl. 68

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Fluvastatin inhibits recruitment of HIV envelope protein-triggered CD+4 T cell to vascular endothelium2004

    • Author(s)
      Y.Takano, K.Shimokado, Y.Hata, M.Yoshida
    • Journal Title

      Circulation J. Suppl. 68

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A phase I/II study of tegafur-uracil(UFT) plus oral leucovorin(IV) and biweekly CPT-11 therapy for patients(pts) with metastatic colorectal cancer(CRC).2004

    • Author(s)
      Uetake H
    • Journal Title

      JOURNAL OF CLINICAL ONCOLOGY 22(14): Suppl.S, JUL 15

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary

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Published: 2003-04-01   Modified: 2016-04-21  

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