| Project/Area Number |
15591388
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
YAMADA Hiroyuki (2004) Tokyo Medical and Dental University, Faculty of Medicine, assistant, 医学部附属病院, 助手 (70313244)
山下 俊樹 (2003) 東京医科歯科大学, 医学部附属病院, 助手 (80334415)
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| Co-Investigator(Kenkyū-buntansha) |
UETAKE Hiroyuki Tokyo Medical and Dental University, Faculty of Medicine, assistant, 医学部附属病院, 助手 (60311651)
OSANAI Takeyuki Tokyo Medical and Dental University, Graduate School, assistant, 学院医歯学総合研究科, 助手 (50301164)
KOJIMA Kazuyuki Tokyo Medical and Dental University, Graduate School, lecturer, 大学院・医歯学総合研究科, 講師 (60313243)
SUGIHARA Kenichi Tokyo Medical and Dental University, Graduate School, professor, 大学院・医歯学総合研究科, 教授 (10171167)
山田 博之 東京医科歯科大学, 医学部附属病院, 助手 (70313244)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Tissue Microarray / immunity histology experiment / Donor block / Array block / Recipient block / cancer-related protein |
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| Research Abstract |
Forty-one gastric cancer specimens (20 were differentiated adenocarinoma and 21 undifferentiated) were analyzed in this study. Using Manual tissue arrayer, 2 grafts (2mm in diameter), which showed representative histological feature, were taken out as from each specimen ("donor block"). Forty-eight grafts were put per "recipient block". The graft was lined up in the donor block by histological type and tumor stage. In cutting the recipient block, successfully cut into 3 micro-meter thickness without the grafts in the recipient block being destroyed. It became clear that an array block well reflected the histological structure of donor blocks in tumor cell and gland density when it was compared with donor blocks. In immunohistochemistory, a positive correlation was observed in p-53, Ki-57 and COX-2 expression intensity between donor and microarray (p<0.0005, <0.0005 and <0.0001, respectively). Therefore, diagnostic system by immunohistochemistory using Tissue Microarray was supposed to be useful for human gastric cancer
We are now evaluating the efficacy of Tissue Microarray by analyzing the expression of other proteins, such as VGEF-C, thymidylate synthase, dihydropyrimidine dehydrogenase, thymidine phosphorylase and so on..
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