| Project/Area Number |
15591414
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Nagasaki University |
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Principal Investigator |
ENJOJI Akihito Nagasaki University, Graduate School of Biomedical Sciences, Assistant Professor, 大学院・医歯薬学総合研究科, 講師 (10295066)
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| Co-Investigator(Kenkyū-buntansha) |
KANEMATSU Takashi Nagasaki University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (40128004)
TANIYAMA Kohtaro Nagasaki University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (70030898)
NAGATA Yasuhiro Nagasaki University, Hospital of Medicine and Dentistry, Assistant Professor, 医学部・歯学部附属病院, 講師 (80336164)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | ghrelin / motility / microdialysis / asetylcholine / strain gauge force transducer / マイクロダイアリシス法 |
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| Research Abstract |
[background]The intestinal motor activity was physiologically associated mainly with cholinergic neural activity which was regulated by cholinergic neuron itself and adrenergic neuronal, in the whole body animal. Application of microdialysis to gastrointestinal tissue may offer a potential advantage over traditional methods using isolated preparations because it allow s for continuous, long-term sampling of intestinal solute concentration within the region of gastrointestinal tissues in which a dialysis probe has been placed. We apply microdyalysis to alimentary canal by this study and perform action mechanism elucidation of ghrelin in vivo..
We could not observe the contraction of gastrointestinal tract at the time of ghrelin administration by intravenous infusion in a dog model, we made a direct microdialysis model and made a ghrelin intraarterial injection model in a stomach.
[Materias and Methods]The dogs were anesthetized with pentobarbital Na, and surgical procedures were performed. A catheter connected to a injection-syringe was inserted in the marginal gastroepiploic arteries for intraarterial injection of the ghrelin or other drugs. A strain gauge force transducer was then sutured to the serosa of the defined area of the stomach, and contractility in the circular muscle direction was recorded isometrically.
[Results]The contraction was not observed to a stomach in intraarterial injection of ghrelin. A problem of maneuver had the measurement in a gastric wall. We were not able to show the action for a stomach of ghrelin confirmed with rats.
We review a change of measurement locus [a future problem] and think a ghrelin administration model to be need a change of experimental animal.
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