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Analyses for new genes related to invasion and metastases of human pancreatic cancer cell

Research Project

Project/Area Number 15591415
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionUniversity of Miyazaki (2004)
宮崎医科大学 (2003)

Principal Investigator

JIMI Sei-ichiro (2004)  University of Miyazaki, Dept.Surgery 1, Assistant Professor, 医学部, 助手 (90363598)

岩村 威志 (2003)  宮崎大学, 医学部, 助教授 (50151759)

Co-Investigator(Kenkyū-buntansha) ETO Tadaaki  University of Miyazaki, Dept.Surgery 1, Assistant Professor, 医学部, 助手 (30322316)
自見 政一郎  宮崎大学, 医学部, 助手 (90363598)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥2,600,000 (Direct Cost: ¥2,600,000)
KeywordsPancreatic cancer cell / metastasis invasion / Thrombin / PAR-1 / Vitronectin / ヒト膵癌細胞 / PAR1 / P-セレクチン
Research Abstract

1)Proteinase-activated receptor-1(PAR-1) has been reported to be related to the invasion and metastasis. PAR-1 is a G-protein coupled, which couples 7 times to a trans-membrane type receptor. The N-terminal extracellular component of PAR-1 was cleaved by thrombin and PAR-1 was activated by the self-binding of the N-terminal component. The mRNA expression of PAR-1 on cancer cells and its biological significance is still not clear. We examined the mRNA expression of PAR-1 by real time-PCR in SUIT-2 human pancreatic cancer cell line and its sublines, which were established in our laboratory. We examined both cell growth and cell adhesion to the extracellular matrix of these cell lines by an MTT assay. We studied the effect of a PAR-1 agonist, TRAP-6, and a PAR-1 antagonist, SCH79797, on the cell adhesion to vitronectin. The mRNA expression of PAR-1 was confirmed in all our cell lines. The cell growth and cell adhesion were enhanced by thrombin in almost all cell lines, while they were activated by TRAP-6 and inhibited by SCH79797. The effect of thrombin activation on PAR-1 was found to be related to both pancreatic cancer cell growth and cell adhesion. We have a plan to perform further analyses to the mechanisms of invasion and metastases of these cell lines.
2)We established a new human pancreatic cancer cell line, named SUIT-4. This cell line formed a subcutaneous tumor in the nude mouse, which was histologically moderately differentiated adenocarcinoma. Six of 21 mice demonstrated lymph node metastasis and 4 showed lung metastases. This cell line also had a high metastatic potential (Ref.1). We intend to analyze the molecular mechanisms of invasion and metastases in these cell lines.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (13 results)

All 2004 2003

All Journal Article (13 results)

  • [Journal Article] Establishment and characterization of a novel human pancreatic cancer cell line (SUIT-4) metastasizing to lymph nodes and lungs in nude mice.2004

    • Author(s)
      Kawano, K., et al.
    • Journal Title

      Oncology 66

      Pages: 458-467

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Establishment and characterization of a novel human pancreatic cancer cell line (SUIT-4) metastasizing to lymph nodes and lungs in nude mice.2004

    • Author(s)
      Kawano, K., et al.
    • Journal Title

      Oncology 66(6)

      Pages: 458-467

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Establishment and characterization of a novel human pancreatic cancer cell line (SUIT-4) metastasizing to lymph nodes and lungs in nude mice.2004

    • Author(s)
      Kawano, K., Iwamura, T., et al.
    • Journal Title

      Oncology 66・6

      Pages: 458-467

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Selective activation of PPAR γ inhibits pancreatic cancer invasion and decreases expression of tissue plasminogen activator.2003

    • Author(s)
      Farrow, B., et al.
    • Journal Title

      Surgery 134

      Pages: 206-212

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Inhibition of pancreatic cancer cell growth and induction of apoptosis with novel therapies directed against protein kinase A.2003

    • Author(s)
      Farrow, B., et al.
    • Journal Title

      Surgery 134

      Pages: 197-205

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] SERPINE2 (protease nexin I) promotes extracellular matrix production and local invasion of pancreatic tumors in vivo.2003

    • Author(s)
      Buchholz, M., et al.
    • Journal Title

      Cancer Research 63

      Pages: 4945-4951

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Claudin-4 expression decreases invasiveness and metastatic potential of panreatic cancer.2003

    • Author(s)
      Michl, P., et al.
    • Journal Title

      Cancer Research 63

      Pages: 6265-6271

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Selective activation of PPARγ inhibits pancreatic cancer invasion and decreases expression of tissue plasminogen activator.2003

    • Author(s)
      Farrow, B., et al.
    • Journal Title

      Surgery 134

      Pages: 206-212

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Claudin-4 expression decreases invasiveness and metastatic potential of pancreatic cancer.2003

    • Author(s)
      Michl, R., et al.
    • Journal Title

      Cancer Research 63

      Pages: 6265-6271

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Selective activation of PPAR γ inhibits pancreatic cancer invasion and decreases expression of tissue plasminogen activator.2003

    • Author(s)
      Farrow, B., Iwamura, T., et al.
    • Journal Title

      Surgery 134・2

      Pages: 206-212

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Inhibition of pancreatic cancer cell growth and induction of apoptosis with novel therapies directed against protein kinase A.2003

    • Author(s)
      Farrow, B., Iwamura, T., et al.
    • Journal Title

      Surgery 134・2

      Pages: 197-205

    • Related Report
      2004 Annual Research Report
  • [Journal Article] SERPINE2 (protease nexin I) promotes extracellular matrix production and local invasion of pancreatic tumors in vivo.2003

    • Author(s)
      Buchholz, M., Iwamura, T., et al.
    • Journal Title

      Cancer Research 63・16

      Pages: 4945-4951

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Claudin-4 expression decreases invasiveness and metastatic potential of pancreatic cancer.2003

    • Author(s)
      Michl, P., Iwamura, T., et al.
    • Journal Title

      Cancer Research 63・19

      Pages: 6265-6271

    • Related Report
      2004 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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