| Project/Area Number |
15591420
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | First Department of Surgery, School of medicine, Sapporo Medical University |
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Principal Investigator |
KATSURAMAKI Tadashi Sapporo Medical University, School of medicine, Assistant professor, 医学部, 助教授 (50253993)
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| Co-Investigator(Kenkyū-buntansha) |
HIRATA Koichi Sapporo Medical University, School of medicine, Professor, 医学部, 教授 (50136959)
KIMURA Yasutoshi Sapporo Medical University, School of medicine, Assistant professor, 医学部, 助手 (80311893)
向谷 充宏 札幌医科大学, 医学部, 講師 (00253998)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2003: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Liver / Artificial blood / Normothermic perfusion / Ischemia / reperfusion injury / Nitric Oxide / Pig |
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| Research Abstract |
Background. We tried to development of ex vivo hepatic organ culture system, and we have previously demonstrated that development of a normothermic perfusion system that employed artificial products. In this study, we investigated the effects of administration of iNOS inhibitor or NO donor in a normothermic perfusion system with an artificial perfusate using artificial blood.
Materials and Methods. A liver graft from a female pig weighting 15 kg was harvested in the usual manner. The Perfusion solution consisted of artificial blood (Neo Red Cell), L-15 medium, distilled water, bovine serum albumin, NaHCO3, NaOH, KC1, human regular insulin, 50% glucose solution, and Hexamethasone. The isolated liver was perfused with this oxygenated perfusate through the portal vein at a rate of 300 ml/min for 6 hours. Selective iNOS inhibitor or NO donor was administration in this perfusion system.
Results., Serum contents of AST and LDH level in iNOS inhibitor treatment group markedly dropped and improve oxygen consumption level compared with control group. Histological findings showed that few necrotic areas in 3 hour after perfusion in iNOS inhibitor treatment group compared with control group. NO donor administaration in the perfusion solution improved FischerAfs ratio and decrease cytotoxic amino acid such as serine and methionine. Conclusions. A selective iNOS inhibitor inhibited isolated liver injury. NO donor administration contributed to improvement of amino acid metabolism.
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