| Project/Area Number |
15591423
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
SAITO Takuro Fukushima Medical University, Department of Surgery I, Medical Staff, 医学部, 助手 (20305361)
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| Co-Investigator(Kenkyū-buntansha) |
TSUCHIYA Takao Fukushima Medical University, Department of Surgery I, Medical Staff, 医学部, 助手 (70343390)
SATOH Yoshihiro Fukushima Medical University, Department of Surgery I, Medical Staff, 医学部, 助手 (60347218)
GOTOH Mtsukazu Fukushima Medical University, Department of Surgery I, Professor, 医学部, 教授 (50162160)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 2003: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | liver fibrosis / bile duct ligation model / bone marrow transplantation / Green mouse / anti-GFP antibody |
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| Research Abstract |
BACKGROUND/AIMS : Bone marrow derived cells are involved in the process of hepatic fibrosis secondary to chronic injury. However, it is not yet known how quickly this event occurs in acute fibrosis models. The purpose of this study was to determine the role of bone marrow cells in rapid fibrosis following bile duct ligation in mice using chimeric green fluorescent protein expressing bone marrow cells.
METHODOLOGY : After whole body irradiation, 1 x 10^6 donor whole bone marrow cells from green fluorescent protein^<+/-> mice were transplanted into C57BL/6 recipients via the tail vein. Four weeks after bone marrow transplantation, chimeric mice were subjected to common bile duct ligation, and livers of these animals were histologically examined after bile duct ligation using anti-fibroblast specific protein-1 antibody and anti-alpha-smooth muscle actin antibody.
RESULTS : Periductal fibrosis consisting of fibroblast specific protein-positive cells was demonstrated histologically as early as day 7. Most of the fibrotic cells were green fluorescent protein-negative, however, a significant number of cells (10%) were green fluorescent protein-positive and some were also anti-fibroblast specific protein-1-or alpha-smooth muscle actin-positive.
CONCLUSIONS : Differentiation of bone marrow derived cells to activated fibroblast and myofibroblast-like phenotypes occurs in the very early course of periductal fibrosis following bile duct ligation.
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