| Project/Area Number |
15591428
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
OTSUJI Eigo Kyoto Prefectural University of Medicine, School of Medicine, Department of Surgery, Assistant Professor, 医学研究科, 講師 (20244600)
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| Co-Investigator(Kenkyū-buntansha) |
OKAMOTO Kazuma Kyoto Prefectural University of Medicine, Department of Surgery, Assistant Professor, 医学研究科, 助手 (20285258)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | GADRINIUM / MNOCLONAL ANTIBODY / MR imaging / contrast agent / 膵癌 |
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| Research Abstract |
Local disease is the most frequent recurrence pattern of rectal carcinoma, and its prognosis is not good. To detect local recurrence as early as possible, we produced the monoclonal antibody A7-gadolinium(Mab A7-Gd) conjugate as a contrast agent for magnetic resonance imaging to distinguish between carcinoma and normal tissue. We examined the in vitro immunoreactivity of Mab A7 coupled to Gd by chelate, isothiocyanobenzyl EDTA and its in vivo distribution in nude mice with human colorectal carcinoma. Mab A7-Gd retained binding activities that were nearly identical to intact Mab A7. More radiolabelled Mab A7-Gd accumulated in the tumour than normal mouse IgG-Gd. Both Mab A7-Gd and normal mouse IgG-Gd disappeared from blood linearly over time. Accumulation levels in normal tissues decreased linearly over time but were lower than those in tumours. Mab A7 conjugated to gadolinium is potentially suitable as a contrast agent for MR imaging of local rectal carcinoma recurrence.
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