Usefulness of BIACORE technology for the development of telomere/telomerase-targeted therapies

• Project/Area Number: 15591432
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: Iwate medical University School of Medicine
• Principal Investigator: SHIDA Kaorul Iwate Med. Univ. School of Med., Dept. of Surg., Assistant Professor, 医学部, 助教授 (70146041)
• Co-Investigator(Kenkyū-buntansha): MAESAWA Chihaya Iwate Med. Univ. School of Med., Dept. of Surg., Assistant Professor, 医学部, 助教授 (10326647)
• Project Period (FY): 2003 – 2004
• Project Status: Completed (Fiscal Year 2004)
• Budget Amount: ¥3,800,000 (Direct Cost: ¥3,800,000); Fiscal Year 2004: ¥500,000 (Direct Cost: ¥500,000); Fiscal Year 2003: ¥3,300,000 (Direct Cost: ¥3,300,000)
• Keywords: Stem cell / hTERT / TRE assay / Replicative senescence / Estradiol / Biomolecular ineraction / Telomere / Telomerase / テロメラーゼ / テロメア / ARK / G-quardplex / 分子標的治療薬 / 分子標的治療 / TRE assay / 生体内分子間相互作用解析装置

Research Abstract

The telomere is a special cap? like structure at the end of eukaryotic chromosomes, composed of tandem TTAGGG repeats. Telomere length is maintained by a balance between two mechanism: elongation (telomerase) and shortening (end-replication problem) of hexameric repeats. We think that telomerase and telomere structure become potentially important treatment targets for cancer therapy. We have recently developed the telomeric repeat elongation (TRE) assay, a rapid biosensor chip assay using surface plasmon resonance (SPR), for the quantitative assessment of telomerase activity. We have recently focused on the telomerase catalytic subunit (human telomerase reverse transcriptase: hTERT) as a target of telomerase therapy. It is well known that estrogen activates telomerase via a direct effect on the hTERT promoter. In fact, breast cancer cell lines have greater telomerase activity than other types of cancer cell lines. We evaluated the inhibitory effects of tamoxifen (TAM, an anti-estrogen agent), which acts competitively on the estrogen response element (ERE) at the hTERT promoter. TAM decreased the telomerase activity of breast cancer cell lines by approximately 50%, although the down-regulated level was insufficient to achieve cellular senescence or crisis. We have also applied the BIACORE apparatus for screening telomerase inhibitors. The G?quadruplex structure is a suitable target for telomere/telomerase?targeted therapy in the development of a new cancer treatment strategy. To investigate the inhibitory effects of G-quadruplex-mediated telomerase inhibitors, we performed a kinetic assay using the BIACORE apparatus. Rate constants for the interaction of each compound with telomeric DNA were well correlated with the inhibitory effects of telomerase activity evaluated by the TRE assay. Kinetic and TRE assays using SPR technology will be useful for seeking new telomerase inhibitors for molecular target therapy.

Research Products

• [Journal Article] Epigenetic status and aberrant expression of the maspin gene in human hepato-biliary tract carcinomas. (2005)
  • Author(s): Fujisawa K, Maesawa C, Sato R, Wada K, Ogasawara S, Akiyama Y, Takeda M, Fujita T, Otsuka K, Higuchi T, Suzuki K, Saito K, Masuda T
  • Journal Title: Lab Invest 85 Pages: 214-224
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Consensus JH gene probes with conjugated 3'-minor groove binder for monitoring minimal residual disease in acute lymphoblastic leukemia. (2005)
  • Author(s): Uchiyama M, Maesawa C, Yashima-Abo A, Tarusawa M, Endo M, Sugawara W, Chida S, Onodera S, Tsukushi Y, Ishida Y, Tsuchiya S, Masuda T.
  • Journal Title: J Mol Diagn 7 Pages: 121-126
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Expression of human telomerase reverse transcriptase splice variants is well correlated with low telomerase activity in osteosarcoma cell lines. (2005)
  • Author(s): Fujiwara-Akita H, Maesawa C, Honda T, Kobayashi S, Masuda T.
  • Journal Title: Int J Oncol 26 Pages: 1009-1016
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Infrequent mutation of the human envoplakin gene is closely linked to the tylosis oesophageal cancer locus in sporadic oesophageal squamous cell carcinomas. (2005)
  • Author(s): Iwaya T, Maesawa C, Kimura T, Ogasawara S, Ikeda K, Kimura Y, Noda Y, Ishida K, Sato N, Saito K, Masuda T.
  • Journal Title: Oncol Rep 13 Pages: 703-707
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Epigenetic status and aberrant expression of the maspin gene in human hepato-biliary tract carcinomas. (2005)
  • Author(s): Fujisawa K, Maesawa C, Sato R, Wada K, Ogasawara S, Akiyama Y, Takeda M, Fujita T, Otsuka K, Higuchi T, Suzuki K, Saito K, Masuda T.
  • Journal Title: Lab Invest. Feb 85(2) Pages: 214-24
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Consensus JH gene probes with conjugated 3'-minor groove binder for monitoring minimal residual disease in acute lymphoblastic leukemia. (2005)
  • Author(s): Uchiyama M, Maesawa C, Yashima-Abo A, Tarusawa M, Endo M, Sugawara W, Chida S, Onodera S, Tsukushi Y, Ishida Y, Tsuchiya S, Masuda T.
  • Journal Title: J Mol Diagn. Feb;7(1) Pages: 121-6
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression of human telomerase reverse transcriptase splice variants is well correlated with low telomerase activity in osteosarcoma cell lines (2005)
  • Author(s): Fujiwara-Aikita H, Maesawa C, Honda T, Kobayashi S, Masuda T.
  • Journal Title: Int J Oncol. Apr 26(4) Pages: 1009-16
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Infrequent mutation of the human envoplakin gene is closely linked to the tylosis oesophageal cancer locus in sporadic oesophageal squamous cell carcinomas. (2005)
  • Author(s): Iwaya T, Maesawa C, Kimura T, Ogasawara S, Ikeda K, Kimura Y, Noda Y, Ishida K, Sato N, Saito K, Masuda T.
  • Journal Title: Oncol Rep. Apr 13(4) Pages: 703-7
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression of human telomerase reverse transcriptase splice variants is well correlated with low telomerase activity in osteosarcoma cell lines. (2005)
  • Author(s): Fujiwara-Akita H, Maesawa C, Honda H, Kobayashi S, Masuda T
  • Journal Title: Int J Oncol 26・4 Pages: 1009-1016
• [Journal Article] Infrequent mutation of the human envoplakin gene closely linked to the tylosis oesophageal cancer (TOC) locus in sporadic oesophageal squamous cell carcinomas. (2005)
  • Author(s): Iwaya T, Maesawa C.Uesugi N, Kimura T, Ogasawara, Ikeda K, Kimura Y, Mitomo S, Ishida K, Sato N, Saito K, Masuda T.
  • Journal Title: Oncol Rep 13・4 Pages: 703-707
• [Journal Article] Expression and localization of tumour necrosis factor-alpha and its converting enzyme in human abdominal aortic aneurysm. (2004)
  • Author(s): Satoh H, Nakamura M, Satoh M, Nakajima T, Izumoto H, Maesawa C, Kawazoe K, Masuda T, Hiramoti K.
  • Journal Title: Clin Sci (Lond) 106 Pages: 301-306
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Development of immunoglobulin variable heavy chain gene consensus probes with conjugated 3' minor groove binder groups for monitoring minimal residual disease in childhood acute lymphoblastic leukaemia. (2004)
  • Author(s): Uchiyama M, Maesawa C, Yashima A, Itabashi T, Satoh T, Tarusawa M, Endo M, Takahashi Y, Sasaki S, Tsuchiya S, Ishida Y, Masuda T.
  • Journal Title: J Clin Pathol 56 Pages: 952-955
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Surgery plus chemotherapy compared with surgery alone for localized squamous cell carcinoma of the thoracic esophagus : a Japan Clinical Oncology Group Study-JCOG 9204. (2004)
  • Author(s): Ando N, Iizuka T, Ide H, Ishida K, Shinoda M, Nishimaki T, Takiyama W, Watanabe H, Isono K, Aoyama N, Makuuchi H, Tanaka O, Yamana H, Ikeuchi S, Kabuto T, Nagai K, Shimada Y, Kinjo Y, Fukuda H, Japan Clinical Oncology Group.
  • Journal Title: J Clin Oncol 21 Pages: 4592-4596
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Disruption of cell-type-specific methylation at the Maspin gene promoter is frequently involved in undifferentiated thyroid cancers. (2004)
  • Author(s): Ogasawara S, Maesawa C, Yamamoto M, Akiyama Y, Wada K, Fujisawa K, Higuchi T, Tomisawa Y, Sato N, Endo S, Saito K, Masuda T.
  • Journal Title: Oncogene 523 Pages: 1117-1124
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Quantitative detection of mutant alleles of the K-ras gene with minor groove binder-conjugated fluorogenic DNA probes. (2004)
  • Author(s): Itabashi T, Maesawa C, Uchiyama M, Higuchi T, Masuda T.
  • Journal Title: Int J Oncol 24 Pages: 687-696
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Human PinX1,a patent telomerase inhibitor, is not involved in human gastrointestinal tract carcinoma. (2004)
  • Author(s): Akiyama Y, Maesawa C, Wada K, Fujisawa K, Itabashi T, Noda Y, Honda T, Sato N, Ishida K, Takagane A, Saito K, Masuda T.
  • Journal Title: Oncol Rep.2004 Apr;11(4):871-4. 11 Pages: 871-874
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Short consensus probes with 3'-minor groove binder of the immunoglobulin heavy-chain gene for real-time quantitative PCR in B-cell non-Hodgkin lymphomas. (2004)
  • Author(s): Uchiyama M, Maesawa C, Yashima-Abo A, Tarusawa M, Satoh M, Satoh T, Ishida Y, Ito S, Murai K, Enomoto S, Utsugisawa T, Masuda T.
  • Journal Title: Lab Invest.2004 Jul;84(7):932-6. 84 Pages: 932-936
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Prognostic significance of peritoneal minimal residual disease in gastric cancer detected by reverse transcription-polymerase chain reaction. (2004)
  • Author(s): Oyama K, Terashima M, Takagane A, Maesawa C.
  • Journal Title: Br J Surg.2004 Apr;91(4):435-43. 91 Pages: 435-443
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Aberrant expression of the maspin gene associated with epigenetic modification in melanoma cells. (2004)
  • Author(s): Wada K, Maesawa C, Akasaka T, Masuda T.
  • Journal Title: J Invest Dermatol 122 Pages: 805-811
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Acute renal failure due to leukemic cell infiltration followed by relapseat multiple extramedullary sites in a child with acute lymphoblastic leukemia. (2004)
  • Author(s): Sato A, Imaizumi M, Shikaoka S, Niizuma H, Hoshi Y, Takeyama J, Fuji K, Nishio T, Watanabe M, Maesawa C, Hayashi Y, Iinuma K.
  • Journal Title: Leuk Lymphoma 45 Pages: 825-828
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Prevention of critical telomere shortening by oestradiol in human normal hepatic cultured cells and carbon tetrachloride injured rat liver fibrosis. (2004)
  • Author(s): Sato R, Maesawa C, Fujisawa K, Wada K, Oikawa K, Takikawa Y, Suzuki K, Oikawa H, Ishikawa K, Masuda T.
  • Journal Title: Gut 53 Pages: 1001-1009
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Phase II study of cisplatin and 5-fluorouracil with concurrent radiotherapy in advanced squamous cell carcinoma of the esophagus : a Japan Esophageal Oncology Group (JEOG) /Japan Clinical Oncology Group trial (JCOG9516). (2004)
  • Author(s): Ishida K, Ando N, Yamamoto S, Ide H, Shinoda M.
  • Journal Title: Jpn J Clin Oncol.2004 Oct;34(10):615-9. 34 Pages: 615-619
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Expression and localization of tumour necrosis factor-alpha and its converting enzyme in human abdominal aortic aneurysm. (2004)
  • Author(s): Satoh H, Nakamura M, Nakajima T, Izumoto H, Maesawa C, Kawazoe K, Masuda T, Hiramori K.
  • Journal Title: Clin Sci (Lond). Mar 106(3) Pages: 301-6
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Disruption of cell-type-specific methylation at the Maspin gene promoter is frequently involved in undifferentiated thyroid cancers. (2004)
  • Author(s): Ogasawara S, Maesawa c, Yamamoto M, Akiyama Y, Wada K, Fujisawa K, Higuchi T, Tomisawa Y, Sato N, Endo S, Saito K, Masuda T.
  • Journal Title: Oncogene. Feb 5:23(5) Pages: 1117-24
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Quantitative detection of mutant alleles of the K-ras gene with minor groove binder-conjugated fluorogenic DNA probes. (2004)
  • Author(s): Itabashi T, Maesawa C, Uchiyama M, Higuchi T, Masuda T.
  • Journal Title: Int J Oncol. Mar24(3) Pages: 687-96
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Human PinX1, a potent telomerase inhibitor, is not involved in human gastrointestinal tract carcinoma. (2004)
  • Author(s): Akiyama Y, Maesawa C, Wada K, Fujisawa K, Itabashi T, Noda Y, Honda T, Sato N, Ishida K, Takagane A, Saito K, Masuda T.
  • Journal Title: Oncol Rep. Apr,11(4) Pages: 871-4
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Short consensus probes with 3'-minor groove binder of the immunoglobulin heavy-chain gene for real-time quantitative PCR in B-cell non-Hodgkin lymphomas. (2004)
  • Author(s): Uchiyama M, Maesawa C, Yashima-Abo A, Tarusawa M, Satoh M, Satoh T, Ishida Y, Ito S, Murai K, Enomoto S, Utsugisawa T, Masuda T.
  • Journal Title: Lab Invest. Jul,84(7) Pages: 932-6
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Prognostic significance of peritoneal minimal residual disease in gastric cancer detected by reverse transcription-polymerase chain reaction. (2004)
  • Author(s): Oyama K, Terashima M, Takagane A, Maesawa C.
  • Journal Title: Br J Surg. Apr,91(4) Pages: 435-43
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Aberrant expression of the maspin gene associated with epigenetic modification in melanoma cells. (2004)
  • Author(s): Wada K, Maesawa C, Akasaka T.
  • Journal Title: J Invest Dermatol. Mar,122(3) Pages: 805-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Acute renal failure due to leukemic cell in filtration followed by relapse at multiple extramedullory sites in a child with acute lymphoblastic leukemia. (2004)
  • Author(s): Sato A, Imaizumi M, Chikaoka S, Niizuma H, Hoshi Y, Takeyama J, Fuji K, Nishio T, Watanabe M, Maesawa C, Hayashi Y, Iinuma K.
  • Journal Title: Leuk Lymphoma. Apr 45(4) Pages: 825-8
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Prevention of critical telomere shortening by oestradiolin human normal hepatic cultured cells and carbon tetrachloride induced rat liver fibrosis. (2004)
  • Author(s): Sato R, Maesawa C, Fujisawa K, Wada K, Oikawa K, Takikawa Y, Suzuki K, Oikawa H, Ishikawa K, Masuda T.
  • Journal Title: Gut. Jul 53(7) Pages: 1001-9
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Phase II study of cisplatin and 5-fluorouracil with concurent radiotherapy in advanced squamous cell carcinoma of the esophagus : a Japan Esophageal Oncology Group (JEOG)/Japan Clinical Oncology Group trial (JCOG9516). (2004)
  • Author(s): Ishida K, Ando N, Yamamoto S, Ide H, Shinoda M.
  • Journal Title: Jpn J Clin Oncol. Oct 34(10) Pages: 615-9
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Disruption of cell-type-specific methylation at the Maspin gene promoter is frequently involved in undifferentiated thyroid cancers. (2004)
  • Author(s): Ogasawara S, Maesawa C, Yamamoto M, Akiyama Y, Wada K, Fujisawa K, Higuchi T, Tomisawa Y, Sato N, Endo S, Saito K, Masuda T.
  • Journal Title: Oncogene 23・5 Pages: 1117-1124
• [Journal Article] Human PinX1, a potent telomerase inhibitor, is not involved in human gastrointestinal tract carcinoma (2004)
  • Author(s): Akiyama Y, Maesawa C, Wada K, Fujisawa K, Itabashi T, Noda Y, Honda T, Sato N, Ishida K, Takagane A, Saito K, Masuda T
  • Journal Title: Oncol Rep 11・4 Pages: 871-874
• [Journal Article] Phase II study of cisplatin and 5-fluorouracil with concurrent radiotherapy in advanced squamous cell carcinoma of the esophagus : a Japan Esophageal Oncology Group (JEOG)/Japan Clinical Oncology Group trial (JCOG9516). (2004)
  • Author(s): Ishida K, Ando N, Yamamoto S, Ide H, Shinoda M.
  • Journal Title: Jpn J Clin Oncol 13・4 Pages: 615-619
• [Journal Article] Quantitative assessment of contaminating tumor cells in autologous peripheral blood stem cells of B-cell non-Hodgkin lymphomas using immunoglobulin heavy chain gene allele-specific oligonucleotide real-time quantitative-polymerase chain reaction. (2003)
  • Author(s): Yashima A, Maesawa C, Uchiyama M, Tarusawa M, Satoh T, Satoh M, Enomoto S, Sugawara K, Numaoka H, Murai K, Utsugisawa T, Ishida Y, Masuda T.
  • Journal Title: Leuk Res 27 Pages: 925-934
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Consensus primers for detecting monoclonal immunoglobulin heavy chain rearrangement in B cell lymphomas. (2003)
  • Author(s): Uchiyama M, Maesawa C, Yashima A, Itabashi T, Ishida Y, Masuda T.
  • Journal Title: J Clin Pathol 56 Pages: 778-779
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Development of consensus flucreogenically labeled probes of the immunoglobulin heavy-chain gene for detecting minimal residual disease in B-cell non-Hodgkin lymphomas. (2003)
  • Author(s): Uchiyama M, Maesawa C, Yashima A, Tarusawa M, Satoh T, Ishida Y, Ito S, Murai K, Enomoto S, Utsugisawa T, Motoyoshi K, Masuda T.
  • Journal Title: Cance Sci 94 Pages: 877-885
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Cell-type-specific repression of the maspin gene is disrupted frequently by demethylation at the promoter region in gastric intestinal metaplasia and cancer cells. (2003)
  • Author(s): Akiyama Y, Maesawa C, Ogasawara S, Terashima M, Masuda T.
  • Journal Title: Am J Pathol 63 Pages: 1911-1919
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Quantitative assessment of contaminating tumor cells in autologous peripheral blood stem cells of B-cell non-Hodgkin lymphomas using immunoglobulin heavy chain gene allele-specific oligonucleotide real-time quantitative-polymerase chain reaction. (2003)
  • Author(s): Yashima A, Maesawa C, Uchiyama M, Tarusawa M, Satoh T, Satoh M, Enomoto S, Sugawara K, Numaoka H, Murai K, Utsugisawa T, Ishida Y, Masuda T.
  • Journal Title: Leuk Res. Oct 27(10) Pages: 925-34
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Consensus primers for detecting monclonal immunoglobulin heavy chain rearrangement in B cell lymphomas. (2003)
  • Author(s): Uchiyama M.Maesawa C, Yashima A, Itabashi T.Ishida Y, Masuda T.
  • Journal Title: J Clin Pathol. Oct 56(10) Pages: 778-9
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Development of consensus fluorogenically labeled probes of the immunoglobulin heavy-chain gene for detecting minimal residual disease in B-cell non-Hodgkin lymphomas. (2003)
  • Author(s): Uchiyama M, Maesawa C, Yashima A, Tarusawa M, Satoh T, Ishida Y, Ito S, Murai K, Enomoto S, Utsugisawa T, Motoyoshi K, Masuda T.
  • Journal Title: Cancer Sci. Oct 94(10) Pages: 877-85
  • NAID: 10012438480
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cell-type-specific repression of the maspin gene is disrupted frequently by demethylation at the promoter region in gastric intestinal metaplasia and cancer cells. (2003)
  • Author(s): Akiyama Y, Maesawa C, Ogasawara S, Terashima M, Masuda T.
  • Journal Title: Am J Pathol. Nov:163(5) Pages: 1911-9
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Development of immunoglobulin variable heavy chain gene consensus probes with conjugated 3' minor groove binder groups for monitaring minimal residual disease childhood acute lymphoblastic leukaemia (2003)
  • Author(s): Uchiyama M, Maesawa C, Yashima A, Itabashi T, Satoh T, Tarusawa M, Endo M, Takahashi Y, Sasaki S, Tsuchiya S, Ishida Y, Masuda T.
  • Journal Title: J Clin Pathol. Dec:56(12) Pages: 952-5
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Japan Clinical Oncology Group. Surgery plus chemotherapy compared with surgery alone for localized squamous cell carcinoma of the thoracic esophagus : a Japan Clinical Oncology Group Study-JCOG9204. (2003)
  • Author(s): Ando N, Iizuka T, Ide H, Ishida K, Shinoda M, Nishimaki T, Takiyama W, Watanabe H, Isono K, Aoyama N, Makuuchi H, Tanaka O, Yamana H, Ikeuchi S, Kabuto T, Nagai K, Shimada Y, Kinjo Y, Fukuda H.
  • Journal Title: J Clin Oncol. Dec 15:21(24) Pages: 4592-6
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Cell-type-specific repression of the maspin gene is disrupted frequently by demethylation at the promoter region in gastric intestinal metaplasia and cancer cells (2003)
  • Author(s): Akiyama Y, Maesawa C, Ogasawara S, Terashima M, Masuda T
  • Journal Title: Am J Pathol 163・5 Pages: 1911-1919
• [Publications] Soto R, Maesawa C, Fujisawa K, Wada K, Oikawa K, Takikawa Y, Suzuki K, Oikawa H, Ishikawa K, Masuda T: "Prevention of critical telomere shortening by estradiol in human normal hepatic cultured cells and CCl4-induced rat liver fibrosis."Gut. 53(in press). (2004)
• [Publications] Uchiyama M, Maesawa C, Yashima A, Tarusawa M, Satoh T, Ishida Y, Ito S, Murai K, Enomoto S, Utsugisawa T, Masuda T: "Short consensus probes with 3'-minor groove binder of the immunoglobulin heavy-chain gene for real-time quantitative PCR in B-cell non-Hodgkin lymphomas."Lab Invest. 84(in press). (2004)
• [Publications] Itabashi T, Maesawa C, Uchiyama M, Higuchi T, Masuda T.: "Quantitative detection of mutant alleles of the K-ras gene with minor groove binder-conjugated fluorogenic DNA probes."Int J Oncol. 24・3. 687-696 (2004)
• [Publications] Akiyama Y, Maesawa C, Wada K, Fujisawa K, Itabashi T, Noda Y, Honda T, Sato N, Ishida K, Takagane A, Saito K, Masuda T.: "Human PinX1, a potent telomerase inhibitor, is not involved in human gastrointestinal tract carcinoma."Oncol Rep. 11・4. 871-874 (2004)
• [Publications] Ogasawara S, Maesawa C, Yamamoto M, Akiyama Y, Wada K, Fujisawa K, Higuchi T, Tomisawa Y, Sato N, Endo S, Saito K, Masuda T: "Disruption of cell-type-specific methylation of the Maspin gene promoter is frequently involved in undifferentiated thyroid cancers."Oncogene. 23・5. 1117-1124 (2004)
• [Publications] Akiyama Y, Maesawa C, Ogasawara S, Terashima M, Masuda T.: "Cell-type-specific repression of the maspin gene is disrupted frequently by demethylation at the promoter region in gastric intestinal metaplasia and cancer cells."Am J Pathol. 163・5. 1911-1919 (2003)