| Project/Area Number |
15591440
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Kyorin University |
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Principal Investigator |
MASAKI Tadahiko Kyorin University, Department of Surgery, Lecturer, 医学部, 講師 (30238894)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Nobutsugu Kyorin University, Department of Surgery, Assistant Professor, 医学部, 助手 (40266747)
MATSUOKA Hiroyoshi Kyorin University, Department of Surgery, Assistant Professor, 医学部, 助手 (60301488)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2005: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Invasion / Metastasis / laminin-5 γ2 chain / Matrix metalloproteinase-7 / adhesion molecule / colon cancer / cohort migration / hepatocyte growth factor / 大腸菌 / 浸潤 / 簇出 / laminin-5 γ2 chain / laminin-5 / Matrix metalloproteinase |
|---|
| Research Abstract |
MMP-7 and laminin-5 γ2 chain expression in cancer cell lines
(1) In L-10 and HT-29 cells, both MMP-7 and laminin-5 γ2 chain were expressed in the cytoplasm of cancer cells, whereas in SW620, Colo320D, LS174T cells these proteins were not expressed.
Cohort migration model
(2) As Nabeshima et al.suggested, cohort migration model was actually observed in L-10 cells, however, it was not observed in the other cell lines examined. L-10 cells formed interlinked, piled-up cell islands on the tissue culture glass slides, and some cancer cells migrated outward from the cell islands into inter-island spaces as coherent cell sheets with one-cell thick. Both MMP-7 and laminin-5 γ2 chain were expressed in the cytoplasm of migrated cancer cells
HGF/SF stimulation test
(3) When stimulated with recombinant hepatocyte growth factor/scattered factor (HGF/SF), the number of migrated L-10 cells were significantly increased as compared with that without stimulation.
Suppression test
(4) When L-10 cells were cultured in the anti-h MMP-7 (+) medium, cohort migration was observed as in the same fashion as when cultured in the ordinary medium. The expression patterns of MMP-7 and laminin-5 γ2 chain were also similar.
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