| Project/Area Number |
15591470
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | Oita University (2004)
大分医科大学 (2003) |
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Principal Investigator |
KAWANO Yozo Oita University, Faculty of medicine, Assistant Professor, 医学部, 助手 (70359793)
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| Co-Investigator(Kenkyū-buntansha) |
WADA Hiromi Kyoto university, Faculty of medicine, Professor, 医学部, 教授 (90167205)
KAWAHARA Katsunobu Oita University, Faculty of medicine, Professor, 医学部, 教授 (80152990)
TANAKA Fumihiro Kyoto university, Faculty of medicine, Senior Assistant Professor, 医学部, 講師 (10283673)
MIURA Takashi Oita University, Faculty of medicine, Senior Assistant Professor, 医学部, 講師 (70295179)
IKENAKA Kazuhiro National Institute for Physiological Science, Professor, 生理学研究所, 教授 (00144527)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | cancer specific antigen / MAGE / lung cancer / 腫瘍 特異 抗原 / MADE |
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| Research Abstract |
This project was aimed to reveal the utility of newly identified gene "MAGE-E1" as the biomarker of diagnosis and treatment of primary lung cancer. Primarily, we revealed that MAGE-E1 was expressed higher in lung cancer than normal lung tissues using RT-PCR and Western blotting. Immunohistochemical staining for MAGE-E1 was performed using paraffin-embedded sections of 180 consecutive patients with p-stage I to IIIA NSCLC, who underwent complete resection without any preoperative therapy. MAGE-E1 was highly expressed at about 60% cases, and its expression was observed frequently in patients with squamous cell carcinoma. The expression level of MAGE-E1 was not correlated with age, sex, tumor differentiation, pathological T factor, N factor and prognosis. Proliferative index of each cases were also examined, and its average score of MAGE-E1 highly expressed group was greater than that of lower expressed group. This result indicates that MAGE-E1 tends to be highly expressed in rapid-growing tumor. In addition, overexpression rate of p53 was also greater in MAGE-E1 highly expressed group. This might be suggested the correlation between MAGE-E1 and tumorgenesis. Lymphatic vessel density(LVD) and microvessel density(MVD) were counted as candidates of prognostic factor. The expression level of MAGE-E1 was significantly correlated with MVD value.
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