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Microscopical analysis of the chromium content in the bronchial and lung tissues of chromate workers and genomic changes of the premalignant lesions.

Research Project

Project/Area Number 15591477
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Thoracic surgery
Research InstitutionThe University of Tokushima

Principal Investigator

KONDO Kazuya  University of Tokushima, Institute of Health Biosciences, senior assistant professor, 大学院・ヘルスバイオサイエンス研究部, 講師 (10263815)

Co-Investigator(Kenkyū-buntansha) MIYOSHI Takanori  University of Tokushima, Institute of Health Biosciences, Assistant Professor, 大学院・ヘルスバイオサイエンス研究部, 助手 (20346612)
UCHIHARA Hiroshi  Horiba Ltd., Kyoto, 分析センター, 研究職
ISHIKAWA Sumiyo  Horiba Ltd., Kyoto, 分析センター, 研究職
門田 康正  徳島大学, 医学部, 教授 (60028628)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordschromate lung cancer / premalignant lesions / chromate accumulation / microscopic X-ray fuorescence analyzer / genomic change / microsatellite instability
Research Abstract

It is known that chromium is an inhaled carcinogen and an important risk factor in the development of lung carcinoma.
METHODS 1 and 2 : The authors used a microscopic X-ray fuorescence analyzer with transmitted X-ray mapping imaging (Horiba, Kyoto, Japan) to measure the accumulation of chromium in 10 resected lung tissue specimens and 90 biopsy specimens from chromate workers. RESULTS 1 and 2 : The maximum chromium accumulation (mean ± standard deviation) in 10 resected lung tissue specimens was 197 ± 238 counts per second (cps)/mili ampere (mA) (range, 4-649 cps/mA). Chromium accumulation was scattered in six tissue specimens and diffuse in one specimen. Chromium accumulation in the proximal bronchi was less than in the bronchioles or subpleural regions of the lung. Chromium accumulation was detectable in 63 (70%) of 90 biopsy specimens, and the mean accumulation was 6.5 ± 9.2 cps/mA (range, 0-46.5 cps/mA). Chromium detected in bronchial tissue specimens was deposited in the bronchial … More stroma but not in the epithelium. The maximum chromium accumulations in dysplastic (n=3), squamous metaplastic (n=10), and normal bronchial epithelia (n=9) in chromate workers and in normal bronchial epithelia (n=3) in non-chromate workers were 20.2 ± 5.4, 18.3 ± 12.2, 13.2 ± 13.4, and 3.0 ± 1.8 cps/mA, respectively. The amount of chromium accumulation significantly increased according to the progression of malignant change of the bronchial epithelium (P=0.003).
METHODS 3 : We examined the replication error (RER) and loss of heterozygosity (LOH) in DNA of dysplastic (n=3), squamous metaplastic (n=10) tissues, using six microsatellite markers containing CA repeats : D3S647 (3p23), D3S966 (3p21.3), D3S1289 (3p21.1), D5S346 (5q21-q22), D9S161 (9p21), and TP53 (17p13.1). The RER phenotype was defined as the presence of microsatellite instability (MSI) at two or more loci. RESULTS 3 : Five (78.9%) of 9 premalignant lesions exhibited RER.
CONCLUSIONS : Considering genetic aberrations and chromium accumulation in these premalignant lesions is useful for elucidating the process of carcinogenesis in chromium-induced lung carcinoma. Less

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (45 results)

All 2005 2004 2003 Other

All Journal Article (44 results) Publications (1 results)

  • [Journal Article] Thymoma and myasthenia gravis : A clinical study of 1320 patients from Japan2005

    • Author(s)
      Kazuya Kondo 他
    • Journal Title

      Ann Thorac Surg 79

      Pages: 219-224

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Microsatellite instability and protein expression of the DNA mismatch repair gene, hMLH1, of lung cancer in chromate-exposed workers2005

    • Author(s)
      Kazuya Kondo 他
    • Journal Title

      Mol Carcinog 42

      Pages: 150-158

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Thymoma and myasthenia gravis : A clinical study of 1320 patients from Japan.2005

    • Author(s)
      Kazuya Kondo
    • Journal Title

      Ann Thorac Surg 79

      Pages: 219-224

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Microsatellite instability and protein expression of the DNA mismatch repair gene, hMLH1, of lung cancer in chromate-exposed workers.2005

    • Author(s)
      Kazuya Kondo
    • Journal Title

      Mol Carcinog 42

      Pages: 150-158

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] WHO histologic classification is a prognostic indicator in thymoma.2004

    • Author(s)
      Kazuya Kondo 他
    • Journal Title

      Ann Thorac Surg 77

      Pages: 1183-1188

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Green fluorescent protein expression visualize mediastinal lymph node metastasis of human lung cancer cell line using orthotopic implantation.2004

    • Author(s)
      Kazuya Kondo 他
    • Journal Title

      Anticancer Res 24

      Pages: 719-724

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Thymidylate Synthase and Dihydropyrimidine Dehydrogenase in Non Small Cell Lung Cancer : Relationship between RNA Expression and Activity2004

    • Author(s)
      Kazuya Kondo 他
    • Journal Title

      Anticancer Res

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Orthotopically implanted SID mice using human lung cancer cell lines and its metastatic patterns.2004

    • Author(s)
      Kazuya Kondo 他
    • Journal Title

      Oncology reports 12

      Pages: 991-999

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Novel bronchofiberscopic catheter spray device allows effective anesthetic spray and sputum suctioning.2004

    • Author(s)
      Kazuya Kondo 他
    • Journal Title

      Respiratory Medicine 98

      Pages: 606-610

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Computed tomography-guide drainage for large pulmonary bullae2004

    • Author(s)
      Kazuya Kondo 他
    • Journal Title

      Interactive Cardiovascular and Thoracic Surgery 3

      Pages: 283-285

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Betulinic acid augments the inhibitory effects of vincristine on growth and lung metastasis of B16F10 melanoma cells in mice.2004

    • Author(s)
      Kazuya Kondo 他
    • Journal Title

      Br J Cancer. 90

      Pages: 1672-1678

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Tree-dimensional imaging of thoracic diseases with 2004. multidetector row CT.2004

    • Author(s)
      Kazuya Kondo 他
    • Journal Title

      J Medical Investigation 51

      Pages: 163-170

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] WHO histologic classification is a prognostic indicator in thymoma.2004

    • Author(s)
      Kazuya Kondo
    • Journal Title

      Ann Thorac Surg 77

      Pages: 1183-1188

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Green fluorescent protein expression visualize mediastinal lymph node metastasis of human lung cancer cell line using orthotopic implantation.2004

    • Author(s)
      Kazuya Kondo
    • Journal Title

      Anticancer Res 24

      Pages: 719-724

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Thymidylate Synthase and Dihydropyrimidine Dehydrogenase in Non Small Cell Lung Cancer : Relationship between RNA Expression and Activity2004

    • Author(s)
      Kazuya Kondo
    • Journal Title

      Anticancer Res (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Orthotopically implanted SID mice using human lung cancer cell lines and its metastatic patterns.2004

    • Author(s)
      Kazuya Kondo
    • Journal Title

      Oncology reports 12

      Pages: 991-999

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Novel bronchofiberscopic catheter spray device allows effective anesthetic spray and sputum suctioning.2004

    • Author(s)
      Kazuya Kondo
    • Journal Title

      Respiratory Medicine 98

      Pages: 606-610

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Computed tomography-guide drainage for large pulmonary bullae.2004

    • Author(s)
      Kazuya Kondo
    • Journal Title

      Interactive Cardiovascular and Thoracic Surgery 3

      Pages: 283-285

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Betulinic acid augments the inhibitory effects of vincristine on growth and lung metastasis of B16F10 melanoma cells in mice.2004

    • Author(s)
      Kazuya Kondo
    • Journal Title

      Br J Cancer. 90

      Pages: 1672-1678

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Tree-dimensional imaging of thoracic diseases with multidetector row CT.2004

    • Author(s)
      Kazuya Kondo
    • Journal Title

      J Medical Investigation 51

      Pages: 163-170

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] WHO histologic classification is a prognostic indicator in thymoma.2004

    • Author(s)
      Kazuya Kondo他
    • Journal Title

      Ann Thorac Surg 77

      Pages: 1183-1188

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Green fluorescent protein expression visualize mediastinal lymph node metastasis of human lung cancer cell line using orthotopic implantation.2004

    • Author(s)
      Kazuya Kondo他
    • Journal Title

      Anticancer Res 24

      Pages: 719-724

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Thymidylate Synthase and Dihydropyrimidine Dehydrogenase in Non Small Cell Lung Cancer : Relationship between RNA Expression and Activity2004

    • Author(s)
      Kazuya Kondo他
    • Journal Title

      Anticancer Res

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Orthotopically implanted SID mice using human lung cancer cell lines and its metastatic patterns.2004

    • Author(s)
      Kazuya Kondo他
    • Journal Title

      Oncology reports 12

      Pages: 991-999

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Novel bronchofiberscopic catheter spray device allows effective anesthetic spray and sputum suctioning.2004

    • Author(s)
      Kazuya Kondo他
    • Journal Title

      Respiratory Medicine 98

      Pages: 606-610

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Computed tomography-guide drainage for large pulmonary bullae2004

    • Author(s)
      Kazuya Kondo他
    • Journal Title

      Interactive Cardiovascular and Thoracic Surgery 3

      Pages: 283-285

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Betulinic acid augments the inhibitory effects of vincristine on growth and lung metastasis of B16F10 melanoma cells in mice.2004

    • Author(s)
      Kazuya Kondo他
    • Journal Title

      Br J Cancer. 90

      Pages: 1672-1678

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Tree-dimensional imaging of thoracic diseases with 2004. multidetector row CT.2004

    • Author(s)
      Kazuya Kondo他
    • Journal Title

      J Medical Investigation 51

      Pages: 163-170

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Fatal air embolism during CT guided pulmonary marking with a hook typed marker.2003

    • Author(s)
      Kazuya Kondo 他
    • Journal Title

      J Thorac Cardiovasc Surg. 126

      Pages: 1207-1209

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Therapy for thymic epithelial tumors. A clinical study of 1320 Patients from Japan2003

    • Author(s)
      Kazuya Kondo 他
    • Journal Title

      Ann Thorac Surg 76

      Pages: 878-885

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Lymphogenous and hematogenous metastasis of thymic epithelial tumors.2003

    • Author(s)
      Kazuya Kondo 他
    • Journal Title

      Ann Thorac Surg 76

      Pages: 1859-1865

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] DNA adducts detected in human gastric mucosa.2003

    • Author(s)
      Kazuya Kondo 他
    • Journal Title

      Cancer Detect Prev 27

      Pages: 209-215

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Increased expression of matrix metalloproteinase 2 and tissue inhibitor of metalloproteinase 2 correlate with poor prognostic variables in thymic epithelial tumors2003

    • Author(s)
      Kazuya Kondo 他
    • Journal Title

      Cancer 98

      Pages: 209-215

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Microscopical analysis of the chromium content in the bronchial and lung tissues of chromate workers.2003

    • Author(s)
      Kazuya Kondo 他
    • Journal Title

      Cancer 98

      Pages: 1822-1829

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Establishment of patient-like SCID mouse model by orthotopically implanting primary cultured cells from surgically-resected lung cancer tissues.2003

    • Author(s)
      Kazuya Kondo 他
    • Journal Title

      Oncology reports 10

      Pages: 1709-1715

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Fatal air embolism during CT guided pulmonary marking with a hook typed marker.2003

    • Author(s)
      Kazuya Kondo
    • Journal Title

      J Thorac Cardiovasc Surg. 126

      Pages: 1207-1209

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Therapy for thymic epithelial tumors. A clinical study of 1320 patients from Japan.2003

    • Author(s)
      Kazuya Kondo
    • Journal Title

      Ann Thorac Surg 76

      Pages: 878-885

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Lymphogenous and hematogenous metastasis of thymic epithelial tumors.2003

    • Author(s)
      Kazuya Kondo
    • Journal Title

      Ann Thorac Surg 76

      Pages: 1859-1865

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] DNA adducts detected in human gastric mucosa.2003

    • Author(s)
      Kazuya Kondo
    • Journal Title

      Cancer Detect Prev. 27

      Pages: 209-215

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Increased expression of matrix metalloproteinase 2 and tissue inhibitor of metalloproteinase 2 correlate with poor prognostic variables in thymic epithelial tumors.2003

    • Author(s)
      Kazuya Kondo
    • Journal Title

      Cancer 98

      Pages: 1822-1829

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Microscopical analysis of the chromium content in the bronchial and lung tissues of chromate workers.2003

    • Author(s)
      Kazuya Kondo
    • Journal Title

      Cancer 98

      Pages: 2420-2429

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Establishment of patient-like SCID mouse model by orthotopically implanting primary cultured cells from surgically-resected lung cancer tissues.2003

    • Author(s)
      Kazuya Kondo
    • Journal Title

      Oncology reports 10

      Pages: 1709-1715

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Microscopical analysis of the chromium content in the chromium-induced malignant and premalignant bronchial lesions of the rat.

    • Author(s)
      Kazuya Kondo 他
    • Journal Title

      Environ Res

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Microscopical analysis of the chromium content in the chromium-induced malignant and premalignant bronchial lesions of the rat.

    • Author(s)
      Kazuya Kondo
    • Journal Title

      Environ Res (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Kazuya Kondo他: "Microscopical analysis of the chromium content in the bronchial and lung tissues of chromate workers"Cancer. 98・11. 2420-2429 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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