| Project/Area Number |
15591488
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thoracic surgery
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| Research Institution | JICHI MEDICAL SCHOOL |
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Principal Investigator |
SATO Yukio Jichi Medical School, Department of Surgery, Assistant professor, 医学部, 講師 (10312844)
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| Co-Investigator(Kenkyū-buntansha) |
SOHARA Yasunori Jichi Medical School, Department of Surgery, Professor, 医学部, 教授 (60114097)
KOBAYASHI Eiji Jichi Medical School, Department of Organ replacement research, Professor, 医学部, 教授 (00245044)
ENDO Shunsuke Jichi Medical School, Department of Surgery, Associate professor, 医学部, 助教授 (10245037)
SUGA Michiharu National Cardiovascular Center, Department of Regenerative Medicine and Tissue Engineering, Laboratory chief, 実験治療開発部・移植免疫研究室, 室長 (80265397)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2003: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Green flurescent protein / Polymorphonuclear leukocyte / Acute lung injury / Nitric oxide / Phoshpodiesterase |
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| Research Abstract |
Activated PMN sequester in pulmonary microvessels and play an important role in acute lung injury. However, the site of PMN sequestration is still controversial, as in vivo real time observation of PMN in pulmonary microvessels has been difficult.
We developed a new method using GFP transgenic rat. Rats were anesthetized, ventilated and catheters were placed in the superior vena cava and the aortic root for the administration of agents and the monitoring of blood pressure. The left anterolateral chest wall was opened, the left lung was stabilized and PMN with GFP was viewed under a fluorescent microscope. The image was obtained with CCD camera and recorded in a hard disk recorder.
Control group (n=4) received the injection of saline. Lipopolysaccaride(LPS) treated group received the injection of LPS (1mg/kg) from Escherichia coli. PMN count decreased in LPS treated group (2610±580 to 1180±220/μl, p<0.05), while PMN count did not change in control (2490±700 to 3320±960/μl). Systolic blood pressure and heart rate did not change in both groups (LPS;121±9 to 123±8mmHg, 319±19 to 295±20/min, control;133±27 to 135±30 mmHg,322±22 to 292±22/min). LPS treatment decreased PMN velocity passing through capillaries from 421±86 to 51±21 μm/sec and increased sequestered PMN in capillaries. The population of rolling leukocytes in postcapillary venule was less than 10 % and LPS treatment did not increase rolling of PMN. Endotoxemia decreased PMN velocity passing through capillaries and increased PMN sequestration in capillaries, but did not change rolling of leukocyte in postcapillary venule. We further evaluated the effect of nitric oxide and phosphodiesterase type 4 inhibitor on PMN sequestration in pulmonary microcirculation.
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