| Project/Area Number |
15591517
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Nagoya University |
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Principal Investigator |
MIYACHI Shigeru Nagoya University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (00293697)
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| Co-Investigator(Kenkyū-buntansha) |
OKAMOTO Shoh Nagoya University, University Hospital, Research Associate, 医学部附属病院, 助手 (10378036)
畠 賢一郎 名古屋大学, 医学部附属病院, 助教授 (80293710)
岡本 剛 名古屋大学, 医学部附属病院, 助手 (00345901)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2003: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | experimental aneurysm / remodeling / cerebral aneurysm / chitosan / hyperthermia / stent / covered stent |
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| Research Abstract |
1)The feasibility of photocrosslinkable chitosan as an embolization material for aneurysms was evaluated. Three experimental aneurysms were created in three Japanese white rabbits. All of the aneurysms were packed with chitosan hydrogel. Histopathologic data were analyzed on two, seven, and 30 days after embolization. Unorganized clots and minimal inflammation around the applied chitosan hydrogel were observed two days after implantation. After seven days, the chitosan was reduced and inflammatory response appeared. At 30 days, most of the aneurysm lumen was replaced with inflammatory cells, and the remaining chitosan was not observed. Severe complications such as anaphylaxis did not occur after the embolization with the chitosan. These results suggest that photocrosslinkable chitosan might be a candidate for an embolization material for endovascular treatment of cerebral aneurysms.
2)Glass model aneurysms packed with coils were subjected to high-frequency altemating magnetic fields(AMF
… More
) to investigate the correlation between weight of platinum and temperature elevation and the correlation between flow rates of water through the model and temperature elevation. Next, activated coagulation time(ACT) of blood obtained from dogs was studies at various temperatures. Finally, side-wall aneurysms created in the canine carotid artery using a venous patch were packed with platinum coils. Change in temperature and angiographic change were investigated after AMF. In the glass model, the weight of platinum was correlated with elevation of temperature, and a negative logarithmic correlation was evident between flow rate and elevation of temperature. Elevation of blood sample temperature tended to shorten ACT. In Canine carotid aneurysms, elevation of intra-aneurysmal temperature was confirmed and sufficient elevation of temperature was found to promote angiographically evident thrombogenesis of the remnant space after AMF application. Local hyperthermia may be useful in completing luminal obliteration of aneurysms after coil embolization. It may particularly usuful for ruptured aneurysms to prevent the early rerupture.
3)Polturethan-coated stent was pplaced in three canine carotid artery resulted in complete thrombosis. Autologus endothelium was implanted on the polyurethan film to reduce the thrombogenisity. Cell culture was succeeded and attached endothelium layer on the film was found not to come off even after loading with various machnical stress like as steretch, rotation and the flow. Less
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