• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of the gene therapy for the malignant brain tumor using the oncolytic herpes virus vector

Research Project

Project/Area Number 15591535
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionKumamoto University

Principal Investigator

NAKAMURA Hideo  Kumamoto University, Medical School Dept.of Neurosurgery, Instructor, 医学部附属病院, 助手 (30359963)

Co-Investigator(Kenkyū-buntansha) MORIOKA Motohiro  Kumamoto University, Medical School Dept.of Neurosurgery, Instructor, 医学部附属病院, 助手 (20295140)
YANO Shigetoshi  Kumamoto University, Medical School Dept.of Neurosurgery, Instructor, 医学部附属病院, 助手 (60332871)
河内 正人  熊本大学, 大学院・医学薬学研究部, 助教授 (70178218)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥900,000 (Direct Cost: ¥900,000)
Keywordsgene therapy / Herpes virus vector / malignant brain tumor / HSV1yCD / 5-FC / 5-FU / cytosine deaminase / Oncolytic virus
Research Abstract

The vector which we have developed was termed HSV1yCD and an oncolytic vector. The most unique character of this virus vector was that it can selectively infect and lyse the tumor cell but not lyse the normal cell. In other words, it can selectively destroy the tumor cells infiltrating into the normal brain. The purpose of this project was to collect the experimental data about the oncolytic virus gene therapy against the brain tumor and to utilize the data for the future clinical gene therapy. Until the year 2003 we have confirmed that HSV1yCD vector can infect the tumor cells and efficiently produce cytosine deaminase which can convert 5-FC to 5-FU. We also have confirmed that 5-FU was effective drug against glioma cells in vitro. In the year 2004 we have challenged the in vivo study using the HSV1yCD. 9L and C6 glioma cells were injected into the rat brains and subsequently the HSV1yCD was injected into the brain. Several experimentally condition has been examined and the optimal experimental methods were established. We have examined the infectious efficacy of the HSV1yCD and the oncolytic activity. Compared the contol vector, HSV1yCD could efficiently destroy the tumor cells. We also have examined the survival rate of the rats injected the tumor cells and subsequently treated with the HSV1yCD and 5-FC. We concluded the HSV1yCD may be a effective vector for the treatment of the brain tumor. Although a further investigation should be required, the HSV1yCD could be a promising tool for the future clinical tool against the brain tumor.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (24 results)

All 2005 2004 Other

All Journal Article (18 results) Publications (6 results)

  • [Journal Article] Dephosphorylation of eNOS on Thr495 after transient forebrain ischemia in gerbil hippocampus2005

    • Author(s)
      Hashiguchi A.
    • Journal Title

      Brain Res Mol Brain Res. 133・2

      Pages: 317-319

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Dephosphorylation of eNOS on Thr495 after transient forebrain ischemia in gerbil hippocampus.2005

    • Author(s)
      Hashiguchi A.
    • Journal Title

      Brain Res Mol Brain Res. 133(2)

      Pages: 317-319

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Functional proteins involved in regulation of intracellular Ca(2+) for drug development : role of calcium/calmodulin-dependent protein kinases in ischemic neuronal death.2005

    • Author(s)
      Yano S.
    • Journal Title

      J Pharmacol Sci. 97(3)

      Pages: 351-354

    • NAID

      10025726712

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Dephosphorylation of eNOS on Thr495 after transient forebrain ischemia in gerbil hippocampus.2005

    • Author(s)
      Hashiguchi A.
    • Journal Title

      Brain Res Mol Brain Res. 133・2

      Pages: 317-319

    • Related Report
      2004 Annual Research Report
  • [Journal Article] The influence of sex and the presence of giant cells on postoperative long-term survival in adult patients with supratentorial glioblastome multiforme.2004

    • Author(s)
      Shinojima N.
    • Journal Title

      J Neurosurg 101・2

      Pages: 219-226

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Combination electro-gene therapy using herpes virus thymidine kinase and interleukin-12 expression plasmids is highly efficient against murine carcinomas in vivo.2004

    • Author(s)
      Goto T.
    • Journal Title

      Mol Ther. 10・5

      Pages: 929-937

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Contribution of angiotensin-converting enzyme and angiotensin II to ischemic stroke : their role in the formation of stable and unstable carotid atherosclerotic plaques.2004

    • Author(s)
      Morioka M.
    • Journal Title

      Surg Neurol. 62・4

      Pages: 292-301

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Ischemia-induced neuronal cell death is mediated by the endoplasmic reticulum stress pathway involving CHOP.2004

    • Author(s)
      Tajiri S.
    • Journal Title

      Cell Death Differ 11・4

      Pages: 403-415

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Oncolysis by viral replication and inhibition of angiogenesis by a replication-conditional herpes simplex virus that expresses mouse endostatin2004

    • Author(s)
      Mullen JT.
    • Journal Title

      Cancer 101・4

      Pages: 869-877

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The influence of sex and the presence of giant cells on postoperative long-term survival in adult patients with supratentorial glioblastoma multiforme.2004

    • Author(s)
      Shinojima N. et al.
    • Journal Title

      J Neurosurg. 101(2)

      Pages: 219-226

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Combination electro-gene therapy using herpes virus thymidine kinase and interleukin-12 expression plasmids is highly efficient against murine carcinomas in vivo.2004

    • Author(s)
      Goto T.
    • Journal Title

      Mol Ther. 10(5)

      Pages: 929-937

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Contribution of angiotensin-converting enzyme and angiotensin II to ischemic stroke : their role in the formation of stable and unstable carotid atherosclerotic plaques.2004

    • Author(s)
      Morioka M.
    • Journal Title

      Surg Neurol. 62(4)

      Pages: 292-301

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Ischemia-induced neuronal cell death is mediated by the endoplasmic reticulum stress pathway involving CHOP.2004

    • Author(s)
      Tajiri S.
    • Journal Title

      Cell Death Differ. 11(4)

      Pages: 403-415

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Oncolysis by viral replication and inhibition of angiogenesis by a replication-conditional herpes simplex virus that expresses mouse endostatin.2004

    • Author(s)
      Mullen JT
    • Journal Title

      Cancer 15.101(4)

      Pages: 869-877

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Selectivity of an oncolytic herpes simplex virus for cells expressing the DF3/MUC1 antigen.2004

    • Author(s)
      Kasuya H
    • Journal Title

      Cancer Res. 1.64(7)

      Pages: 2561-2567

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The influence of sex and the presence of giant cells on postoperative long-term survival in adult patients with supratentorial glioblastoma multiforme.2004

    • Author(s)
      Shinojima N.
    • Journal Title

      J Neurosurg. 101・2

      Pages: 219-226

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Ischemia-induced neuronal cell death is mediated by the endoplasmic reticulum stress pathway involving CHOP.2004

    • Author(s)
      Tajiri S.
    • Journal Title

      Cell Death Differ. 11・4

      Pages: 403-415

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Oncolysis by viral replication and inhibition of angiogenesis by a replication-conditional herpes simplex virus that expresses mouse endostatin.2004

    • Author(s)
      Mullen JT.
    • Journal Title

      Cancer. 101・4

      Pages: 869-877

    • Related Report
      2004 Annual Research Report
  • [Publications] Yamada K. et al.: "Stereotactic biopsy for multifocal, diffuse, and deep-seated brain tumors using Leksell's system"J Glin Neurosci.. 11(3). 263-267 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kamiryo T. et al.: "Correlation between promoter hypermethylation of the O6-methylguanine-deoxyribonucleic acid methyltransferase gene and prognosis in patients with high-grade astrocytic tumors treated with surgery, radiotherapy, and 1-(4-amino-2-methyl-5-pyrimidinyl)methyl-3-(2-chloroethyl)-3-nitrosourea-based chemotherapy"Neurosurgery. 54(2). 349-357 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Shinojima N.et al.: "Prognostic value of epidermal growth factor receptor in patients with glioblastoma multiforme"Cancer Res.. 63(20). 6962-6970 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tada K.et al.: "Preliminary observations on genetic alterations in pilocytic astrocytomas associated with neurofibromatosis 1"Neuro-oncol.. 5(4). 228-234 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Kochi M.et al.: "Successful treatment of intracranial nongerminomatous malignant germ cell tumors by administering neoadjuvant chemotherapy and radiotherapy before excision of residual tumors"J Neurosurg.. 99(1). 104-114 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ushio Y.et al.: "Correlation of molecular genetic analysis of p53, MDM2, p16, PTEN, and EGFR and survival of patients with anaplastic astrocytoma and glioblastoma"Front Biosci.. 1(8). 281-288 (2003)

    • Related Report
      2003 Annual Research Report

URL: 

Published: 2003-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi