| Project/Area Number |
15591558
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Fukuoka University |
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Principal Investigator |
FUKUSIMA Takeo Fukuoka University, School of Medicine, Professor, 医学部, 教授 (10078735)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Masaaki Fukuoka University, School of Medicine, Associate Prof., 医学部, 助教授 (80240125)
NONAKA Masani Fukuoka University, School of Medicine, Assist. Professor, 医学部, 助手 (30352256)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | sonodynamic therapy / focused ultrasound / human glioma cell / nude rat / experimental brain tumor / U87 glioma cell / LRP / horseradish perioxydase / EM study |
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| Research Abstract |
We confirmed efficacy of sonodynamic therapy to the experimental brain tumor, rat C6 glioma which was implanted in Wister rat brain. We evaluated how to effect to the brain tumor and how to effect to the blood brain barrier of the surrounding normal brain tissue using the electron microscope (EM). EM study demonstrated swelling of the cytoplasm, plenty of pinocytosis and interruption of the cytoplasmic membrane in the endothelial cells, suggesting destruction of the blood brain barrier of the peritumoral brain tissue by the sonodynamic therapy using the focused ultrasound. In this experiment, human glioma cells, U251 and U105, which were different in sensitivity to sonodynamic therapy, were used. U251 glioma cells were implanted and survived in the nude rat brain but didn't grow up to neoplastic mass. These cells differed in proliferation depending upon each animal. U 105 glioma cells neither survived in the nude rat brain nor grew up to the mass, so these glioma cells couldnt be used as experimental brain tumor model. We used U87 human glioma cells for experimental brain tumor in the nude rat. These cells were implanted into the nude rat brain successfully and grew up to the mass in a week, so U87 human glioma cells were used for sonodynamic therapy by the focused ultrasound. The nude rat brain was exposed and was irradiated by the focused ultrasound after disinfection, however the lesion was suffered from infection very easily and tended to have meningitis or brain abscess. We are groping to resolve these problems.
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