Clarification of the pathophysiological significance of mast cell tryptase and protease activated receptor-2 (PAR-2) in articular destruction
| Project/Area Number |
15591581
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | The University of Tokushima |
|---|
Principal Investigator |
NAKANO Shunji The University of Tokushima, University Medical and Dental Hospital, Lecturer, 医学部・歯学部附属病院, 講師 (60294683)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NIKAWA Takeshi The University of Tokushima, Graduate School Institute of Health Biosciences, Associate Professor, 大学院・ヘルスバイオサイエンス研究部, 助教授 (20263824)
ENDO Kenji The University of Tokushima, University Medical and Dental Hospital, Lecturer, 医学部・歯学部附属病院, 助手 (60325272)
EGAWA Hiroshi The University of Tokushima, University Medical and Dental Hospital, Lecturer, 医学部・歯学部附属病院, 助手 (20359912)
YASUI Natsuo The University of Tokushima, Graduate School Institute of Health Biosciences, Professor, 大学院・ヘルスバイオサイエンス研究部, 教授 (00157984)
|
|---|
| Project Period (FY) |
2003 – 2004
|
| Project Status |
Completed (Fiscal Year 2004)
|
| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥2,600,000 (Direct Cost: ¥2,600,000)
|
|---|
| Keywords | mast cell tryptase / protease activated receptor / rheumatoid arthritis / osteoarthritis / synovial fibroblast-like cell / IL-8 / synovial fluid / synovium / tryptase / thrombin / cathepsin B / protease-activated receptor / rheumatoid arthritis |
|---|
| Research Abstract |
The purpose of this study is to clarify the pathophysiological significance of mast cell tryptase and protease activated receptor-2 (PAR-2) in articular lesions of patients with rheumatoid arthritis (RA).
We obtained the following results.
1.Trypsin-like activity in synovial fluid was significantly higher in RA than in osteoarthritis (OA) and healthy subjects, while in synovial tissue it was similar in RA and OA.
2.The expression of PAR-2 mRNA in synovial tissue was higher in RA than in OA.
3.The biochemical characteristics of trypsin-like enzyme purified from synovial tissue were similar to those of human mast cell tryptase.
4.Human mast cell tryptase enhanced the proliferation of synovial fibroblast-like cells and the release of IL-8 from these cells.
5.Furthermore, PAR-2 activating peptide stimulated the release of IL-8 from synovial fibroblast-like cells, suggesting that mast cell tryptase in synovial fluid stimulates the proliferation of synovial fibroblast-like cells and the release of IL-8 from these cells via activation of PAR-2.
In summary, trypsin-like activity in synovial fluid was significantly higher in RA than in OA and healthy subjects. Mast cell tryptase and activation of its receptor (PAR-2) enhanced the proliferation of synovial fibroblast-like cells and the release of IL-8 from these cells. Thus, in rheumatoid lesions, mast cell tryptase is involved in amplifying arthritis via PAR-2.
|
Report
(3 results)
Research Products
(20 results)
