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gene therapy for massive bone defects : Development of the delivery system for clinical use

Research Project

Project/Area Number 15591589
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionNagasaki University

Principal Investigator

SHINDO Hiroyuki  Nagasaki University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (30107677)

Co-Investigator(Kenkyū-buntansha) ENOMOTO Hiroshi  Nagasaki University, Graduate School of Biomedical Sciences, Research Associate, 大学院・医歯薬学総合研究科, 助手 (90284679)
OKANO Kunihiko  Nagasaki University, Hospital of Medicine and Dentistry, Research Associate, 医学部・歯学部附属病院, 助手 (70325645)
TSUKAZAKI Tomoo  Nagasaki University, Graduate School of Biomedical Sciences, Associate Professor, 大学院・医歯薬学総合研究科, 助教授 (50315230)
OOTURU Akira  Nagasaki University, Hospital of Medicine and Dentistry, Associate Professor, 医学部・歯学部附属病院, 助教授 (00233198)
山口 朗  長崎大学, 大学院・医歯薬学総合研究科, 教授 (00142430)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsBone defect / gene transfer / bone regeneration / 遺伝子治療 / BMP-2
Research Abstract

There is difficulty in repairing the massive bone defect caused by bone tumor, severe injury, or osteomyelitis. Recently, clinical applications of stimulating factors for bone formation have been expected. However, some problems were reported in gene transfer, such as carcinogenicity or immune reaction. Therefore, we have tried to find the effective gene transfer. We have found that fibroblasts infected with adenovirus vector encoding BMP-2 induced nonosteogenic cells to osteoblasts. We have tested the electroporation via the nucleofector machine, and found that nucleofection technique exhibited a high transfection efficiency. These findings suggest that fibroblasts transfected with BMP-2 induce bone formation, which might be useful for repairing the bone defects.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (20 results)

All 2005 2004 Other

All Journal Article (16 results) Publications (4 results)

  • [Journal Article] Critical regulation of BMP-induced osteoblastic differentiation by delta1/jagged 1-activated notch1 signaling.2005

    • Author(s)
      Nobta M et al.
    • Journal Title

      J Biol Chem. 280・16

      Pages: 15842-15848

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Critical regulation of BMP-induced osteoblastic differentiation by delta1/jagged1-activated notchl signaling.2005

    • Author(s)
      Nobta M et al.
    • Journal Title

      J Biol Chem 280(16)

      Pages: 15842-15848

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Critical regulation of BMP-induced osteoblastic differentiation by deltal/jaggedl-activated notchl signaling.2005

    • Author(s)
      Nobta M et al.
    • Journal Title

      J Biol Chem. (印刷中)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Autocrine/paracrine role of the angiopoietin-1 and -2/Tie2 system in cell proliferation and chemotaxis of cultured Fibroblastic synoviocytes in rheumatoid arthritis.2004

    • Author(s)
      Takahara K et al.
    • Journal Title

      Hum Pathol. 35・2

      Pages: 150-158

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Establishment of a clonal human mesenchymal cell line that retains multilineage differentiation capacity from a spinal hamartoma.2004

    • Author(s)
      Doiguchi Y et al.
    • Journal Title

      Cell Tissue Res 317・3

      Pages: 237-246

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Altered expression of inflammatory cytokines in primary osteoarthritis by human T lymphotropic virus type I retrovirus2004

    • Author(s)
      Yoshihara Y et al.
    • Journal Title

      Arthritis Res Ther. 6・4

      Pages: 347-354

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Histological analysis and biological effects of granulation tissue around loosened hip prostheses in the development of osteolysis.2004

    • Author(s)
      Ito S et al.
    • Journal Title

      Orthop Sci 9・5

      Pages: 478-487

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Bone morphogenetic protein-2 induces differentiation of multipotent C3H10T1/2 cells into osteoblasts, chondrocytes, and adipocytes in vivo and in vitro.2004

    • Author(s)
      Date et al.
    • Journal Title

      J Orthop Sci. 9・5

      Pages: 503-508

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Autocrine/paracrine role of the angiopoietin-1 and -2/Tie2 system in cell proliferation and chemotaxis of cultured fibroblastic synoviocytes in rheumatoid arthritis.2004

    • Author(s)
      Takahara K et al.
    • Journal Title

      Hum Pathol. 35(2)

      Pages: 150-158

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Establishment of a clonal human mesenchymal cell line that retains multilineage differentiation capacity from a spinal hamartoma.2004

    • Author(s)
      Doiguchi Y et al.
    • Journal Title

      Cell Tissue Res 317(3)

      Pages: 237-246

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Altered expression of inflammatory cytokines in primary osteoarthritis by human T lymphotropic virus type I retrovirus2004

    • Author(s)
      Yoshihara Y et al.
    • Journal Title

      Arthritis Res Ther 6(4)

      Pages: 347-354

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Histological analysis and biological effects of granulation tissue around loosened hip prostheses in the development of osteolysis.2004

    • Author(s)
      Ito S et al.
    • Journal Title

      Orthop Sci 9(5)

      Pages: 478-487

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Bone morphogenetic protein-2 induces differentiation of multipotent C3H10T1/2 cells into osteoblasts, chondrocytes, and adipocytes invivo and in vitro.2004

    • Author(s)
      Date et al.
    • Journal Title

      J Orthop Sci. 9(5)

      Pages: 503-508

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Autocrine/paracrine role of the angiopoietin-1 and -2/Tie2 system in cell proliferation and chemotaxis of cultured fibroblastic synoviocytes in rheumatoid arthritis.2004

    • Author(s)
      Takahara K et al.
    • Journal Title

      Hum Pathol. 36・2

      Pages: 150-158

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Establishment of a clonal human mesendhymal cell line that retains multilineage differentiation capacity from a spinal hamartoma.2004

    • Author(s)
      Doiguchi Y et al.
    • Journal Title

      Cell Tissue Res 317・3

      Pages: 237-246

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Bone morphogenetic protein-2 induces differentiation of multipotent C3H10T1/2 cells into osteoblasts, chendrocytes, and adipocytes in vivo and in vitro.2004

    • Author(s)
      Date et al.
    • Journal Title

      J Orthop Sci. 9・5

      Pages: 503-508

    • Related Report
      2004 Annual Research Report
  • [Publications] Iioka et al.: "p300/CBP Acts as a Coactivator to Cartilage Homeoprotein-1 (Cart1), Paired-Like Homeoprotein, via Acetylation of the Conserved Lysine Residue Adjacent to the Homeodomain"J Bone Miner Res. 18・8. 1419-1429 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Taniguchi et al.: "Changes of serum levels of osteocalcin, alkaline phosphatase, IGF-I and IGF-binding protein-3 during fracture healing"Injury. 34・7. 477-479 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Takahara et al.: "Autocrine/paracrine role of the angiopoietin-1 and -2/Tie2 system in cell proliferation and chemotaxis of cultured fibroblastic synoviocytes in rheumatoid arthritis"Hum Pathol. 35・2. 150-158 (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] Uetani et al.: "Subchondral insufficiency fracture of the femoral head : magnetic resonance imaging findings correlated with micro-computed tomography and histopathology"J Comput Assist Tomogr. 27・2. 189-193 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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