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The effect of electrochemotherapy for the lungmetastasis mouse osteosarcoma cell

Research Project

Project/Area Number 15591594
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Orthopaedic surgery
Research InstitutionOsaka city university

Principal Investigator

NAKAMURA Hiroaki  Osaka city university, Orthopaedics, Assistant professor, 大学院・医学研究科, 助教授 (60227931)

Co-Investigator(Kenkyū-buntansha) GOTANI Hiroaki  Osaka city university, University lecturer, 大学院・医学研究科, 講師 (30305627)
TSUZIO Tadao  Osaka city university, Research assistant, 大学院・医学研究科, 助手 (70381994)
TAKAOKA Kunio  Osaka city university, Professor, 大学院・医学研究科, 教授 (30112048)
長山 隆一  大阪市立大学, 大学院・医学研究科, 助手 (50336781)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2005: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 2004: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2003: ¥2,900,000 (Direct Cost: ¥2,900,000)
Keywordsosteosarcoma / electrochemotherapy / electroporation / cancer treatment / methotrexate / drug delivery system / lung metastasis / Dunn osteosarcoma cell / 肺転移
Research Abstract

We clarified the effect of Electrochemotherapy (ECT) with bipolar electrode to the osteosarcoma cell with a mouse. Dunn osteosarcoma cell was transplanted in under the skin with C3H mouse, when tumor grew at 2 week later, we injected Methotrexate (MTX) 10mg/kg into the intraperitonium and executed ECT after 30 minutes. Control group, only MTX group, only electropulse group and ECT group, all divided into 4 groups, we evaluated to the tumor volume increasing rate in time-dependent. The result was shown that 40% tumor reduction in the ECT group at one time execution. Furthermore we changed the direction of electropulse to 4 or 6 directions. When the tumor size was below 5mm, tumor was exterminated but over 5mm tumor cells were locally still alive. Therefore we developed new electrode as multi needle type (9 needles). This device was inserted in the tumor directly with the gel for an electrocardiogram and ECT was done in 50V. The tumor reduced completely whether tumor size was below 5mm or not, but a skin burn was partially recognized. We lowered the voltage to 25V and executed ETC under electropulse frequency of 60 directions. The result was a better and all tumor cells were exterminated, furthermore skin burn was not recognized. Next step we examined the side effect of the ECT to a neurovascular bundle. The mouse model with the Dunn osteosarcoma infiltrated femoral AVN was used and ECT was executed under the same conditions. The result was shown that all tumor cells was death but the influence to a neurovascular bundle was little, the injury of circulation and motor function of leg was not occurred. We have established this method with a mouse model, lung metastasis model with a rabbit as a future schedule consecutively, that would examine and clarify to the curative effect.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (20 results)

All 2005 2004 Other

All Journal Article (15 results) Publications (5 results)

  • [Journal Article] Use of local electroporation enhances methotrexate effects with minimum dose in adjuvant-induced arthritis2005

    • Author(s)
      Tada M, Inui K, Koike T, Takaoka K.
    • Journal Title

      Arthritis & Rheumatism 52

      Pages: 637-641

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Experimental spinal fusion with recombinant human bone morphogenetic Protein-2 delivered by a synthetic polymer and beta-tricalcium phosphate in a rabbit model.2005

    • Author(s)
      Namikawa T, Terai H, Suzuki E, Nakamura H, Takaoka K.
    • Journal Title

      Spine 30

      Pages: 1717-1722

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Repair of an intercalated long bone defect with a synthetic bodegradable bone-inducing implant.2005

    • Author(s)
      Yoneda M, Terai H, Imai Y, Takaoka K.
    • Journal Title

      Biomaterials 26

      Pages: 5145-5152

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Repair of an intercalated long bone defect with a synthetic biodegradable bone-inducing implant.2005

    • Author(s)
      Yoneda M, Terai H, Imai Y, Takaoka K.
    • Journal Title

      Biomaterials 26

      Pages: 5145-5152

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Experimental spinal fusion with recombinant human bone morphogenetic protein-2 delivered by a synthetic polymer and beta-tricalcium phosphate in a rabbit model.2005

    • Author(s)
      Namikawa T, Terai H, Suzuki E, Nakamura H, Takaoka K.
    • Journal Title

      Spine 30

      Pages: 1717-1722

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Does chronic cervical myelopathy affect respiratory function?2005

    • Author(s)
      Toyoda H, Nakamura H, Konishi S, Terai S, Takaoka K.
    • Journal Title

      J Neurosurg Spine 2

      Pages: 175-178

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Use of local electroporation enhances methotrexate effects with minimum dose in adjuvant-induced arthritis2005

    • Author(s)
      Tada M., Inui K., Koike T., Takaoka K.
    • Journal Title

      Arthritis & Rheumatism 52

      Pages: 637-641

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Use of local electroporation enhances methotrexate effects with minimum dose in adjuvant-induced arthritis2005

    • Author(s)
      Tada, M., Inui, K., Koike, T., Takaoka, K.
    • Journal Title

      Arthritis & Rheumatism 52

      Pages: 637-641

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Does chronic cervical myelopathy affect respiratory function?2004

    • Author(s)
      Toyoda H, Nakamura H, Konishi S, Terai S. Takaoka K.
    • Journal Title

      J Neurosurg Spine 2

      Pages: 175-178

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Low-Voltage Electrochemotherapy with Low-Dose Methotrexate Enhances Survival In Mice with Osteosarcoma.2004

    • Author(s)
      Isobe M, Shimizu T, Nikaido T, Takaoka K.
    • Journal Title

      Clinical Orthopaedics and Related Research 426

      Pages: 226-231

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Does chronic cervical myelopathy affect respiratory function?2004

    • Author(s)
      Toyoda, H., Nakamura, H., Konishi, S., Terai, S., Takaoka, K.
    • Journal Title

      J Neurosurg.Spine 2

      Pages: 175-178

    • Related Report
      2004 Annual Research Report
  • [Journal Article] A prostanoid receptor ER4 agonist enhances ectopic bone formation induced by recombinant human bone morphogenetic protein-22004

    • Author(s)
      Sasaoka, R., Terai, H., Toyoda, H., Imai, Y., Sugama, R., Takaoka, K.
    • Journal Title

      Biochemical and Biophysical Research Communication 31

      Pages: 704-709

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Intradural extramedullary hemangioblastoma diffentiated by MR images in the cervical spine2004

    • Author(s)
      Toyoda, H., Seki, M., Nakamura, H., Inoue, Y., Yamano, Y., Takaoka, K.
    • Journal Title

      J Spinal Dis ord.Tech 4

      Pages: 343-347

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Open reduction of the dislocated hip in juvenile idiopathic arthritis : a case report2004

    • Author(s)
      Inui, K., Maeno, T., Tada, M., Takaoka, K., Koike, T.
    • Journal Title

      Mod Rheumatol 14

      Pages: 399-401

    • NAID

      10020420223

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Low-Voltage Electrochemotherapy with Low-Dose Methotrexate Enhances Survival In Mice with Osteosarcoma2004

    • Author(s)
      Isobe, M., Shimizu, T., Nikaido, T., Takaoka, K.
    • Journal Title

      Clinical Orthopaedics and Related Research 426

      Pages: 226-231

    • Related Report
      2004 Annual Research Report
  • [Publications] Nakamura, N., Udagawa, M., Matsuura, S., Takaoka, K., Ozawa, H., Takahashi, N.: "Osteoprotegirin regulates bone formation through a coupling mechanism with bone formation."Endocrinology. 144. 5441-5449 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakamura, Y., Wakitani, S., Nakayama, J., Horiuchi, H., Takaoka, K.: "Temporal and spatial expression of BMPreceptors and noggin suring BMP-2-induced ectopic bone formation"J.Bone and Mineral Research. 18. 1854-1862 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Murakami, G., Watanabe, T., Tkaoka, K., Miyazono, K., Imamura, T.: "Cooperative inhibition of bone morphogenetic protein signaling by smurfl and inhibitory smads"Molecular Biology of the Cell.. 14. 2809-2817 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Saito, N., Horiuchi, H., Takahashi, J., Kojima, Takaoka, K.: "Local bone formation by injection of recombinant human bone morphogenetic protein-2 contained in polymer carreirs"Bone. 23. 381-386 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Murakami N., Saito N., Takahashi, J., Ota, H., Takaoka, K: "Repair of proximal femoral bone defect in dogs using porous sufaced prosthesis in combination with recombinant BMP-2 and a synthetic polymer carrier"Biomaterials. 24. 2153-2159 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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