| Project/Area Number |
15591599
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kitasato University |
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Principal Investigator |
URABE Ken Kitasato University, Dept. of Orthopaedic Surgery, Associate Professor, 医学部, 助教授 (90284489)
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| Co-Investigator(Kenkyū-buntansha) |
NARUSE Kouji Kitasato University, Dept. of Orthopaedic Surgery, Physician Assistant, 医学部, 助手 (60276087)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | umbilical cord blood / chondrocyte / osteoblast / regeneration / cell differentiation / scaffold |
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| Research Abstract |
1.Mesenchymal stem cells are thought to be multipotential, capable of differentiating into multiple lineages. We attempted to characterize rat cells derived from fetal circulating blood (FCBCs) that displayed a fibroblastic morphology and differentiated into osteoblastic and chondrocytic lineages. Notably, they differentiated into a chondrocyte-specific phenotype on plastic culture dishes in medium supplemented only with 10% fetal bovine serum, without the use of a three-dimensional culture substrate. We also determined the adequet harvest period to get more FCBCs and measured the doubling time of FCBCs.
2.Mesenchymal stem cells derived from bone marrow did not express COL2A1 or COL10A1 mRNA under the same culture conditions. FCBCs expressed two splice forms of type II collagen, IIA and IIB. Gene expression of type IIA in FCBCs was more than that in primary cultured rat rib cartilage derived chondrocytes, therefore, FCBCs was thought to be more immature than rib cartilage chondrocytes.
3.Continuous culture promoted the maturation of chondrocytic cells differentiated from FCBCs.
4.Passage culture decreased the ability of spontaneous differentiation of FCBCs.
5.We evaluated the ability of spontaneous differentiation with frozen FCBCs. Frozen FCBCs maintained the ability and differentiated into osteoblastic and chondrocytic cells.
6.FCBCs differentiated into adipogenic cells without 10% fetal bovine serum. These result showed that spontaneous differentiation ability of FCBCs was independent of the factors in the serum.
7.We developed the type II collagen sponge as a scaffold for the repair of osteochondral defect.
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