Mesenchymal stem cell transplantation therapy for one treatment of disc degeneration
| Project/Area Number |
15591605
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Tokai University |
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Principal Investigator |
NOMURA Takeshi ToKai university, school of medicine, Assistant Professor, 医学部, 講師 (60246121)
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| Co-Investigator(Kenkyū-buntansha) |
MOCHIDA Johji Tokai university, school of medicine, Professor, 医学部, 教授 (50174347)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2004: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 2003: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | disc degeneration / nucleus pulposus cells / mesenchymal stem cells / cell transplantation / reproductive medicine / coculture system / clinical application / intervertebral disc / nucleus pulposus / mesenchymal stem cells |
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| Research Abstract |
Study design : using animal disc degeneration model (New Zea Land white rabbits), autologous mesenchymal stem cells (MSCs) bone marrow derived were transplanted into the degenerative disc.
Evaluation : In post operation, the deceleration of disc degeneration was evaluated in various methods, for height of disc by X-ray films, the grade of disc degeneration by MRI films, histological findings of disc by HE and safranine O dyeing, transplanted cell viability by measuring the immunofluorescence of agglycan, type II collagen, chondroitin sulphate, keratin sulphate.
Results : We could confirm that transplanted cells were alive and the effect to the deceleration of disc degeneration was appeared in all evaluative items. And it is suggested that MSCs were differenciated for the chondrocyte-like cells because of expressions of agglycan, type II collagen, chondroitin sulphate, keratin sulphate.
Conclusion : Mesenchymal stem cells might be differentiated for nucleus pulposus cells. Transplantation of mesenchymal stem cells decelerated discdegeneration in vivo
Future Study : We will propose the coculture system between nucleus pulposus cells and mesenchymal stem cells to evaluate the effect of upregulation of the viability of nucleus pulposus cells.
To apply these study for clinical medicine, we will have new experiments such as cell transplantation of nucleus pulposus cells in a large animal model in vivo, culture of human nucleus pulposus cells and mesenchymal stem cells in vitro.
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Report
(3 results)
Research Products
(19 results)
