| Project/Area Number |
15591607
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kurume University |
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Principal Investigator |
ZEMMYO Michihisa Kurume University, School of Medicine, Assistant, 医学部, 助手 (10289465)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAOKA Kohji Kurume University, School of Medicine, Lecturer, 医学部, 講師 (10268914)
FUJII Teruhiko Kurume University, School of Medicine, Assistant, 医学部, 助手 (50199288)
HAMADA Tetsuya Kurume University, School of Medicine, Assistant, 医学部, 助手 (20341349)
WAKIOKA Tohru Kurume University, School of Medicine, Assistant, 医学部, 助手
SHOUDA Takanori Kurume University, School of Medicine, Assistant, 医学部, 助手 (10352163)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2003: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | bone metastasis / breast cancer / signal transduction / MAPK |
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| Research Abstract |
We check the influence which it has on cancer-cells-of-breast-carcinoma proliferation this time while controlling the production of PTHrP and M-CSF by making the dominant negative ERK gene which controls activation of ERK of MAPK pathway overexpression. Moreover, a possibility, negative regulate [IGFs which influences proliferation of a breast carcinoma/SOCS2], is pointed out, and the inhibitory effect of the bone metastasis of the breast carcinoma by making SOCS which controls activation of MAPK simultaneously with activation of JAK/STAT pathway overexpression is checked. Furthermore, the bone metastasis model of an animal is used and it is examined whether bone metastasis can actually be controlled by in vivo in overexpression of the gene of these paralyzer.
Creation of the bone metastasis model according to the ventriclus-sinister injection of MD-MB231 cell using Nude mice Bone metastasis is not started only by injecting MD-MB231 usual cell into the ventriclus sinister. As a result of carrying out using nude mice of 18 animals, it was only one leg that bone metastasis was seen among 18 animals and 72legs. Therefore, MD-MB231 cell was cultured on transmembrane, only the cell which permeates the inferior surface of tongue of a filter was extracted, and this operation was repeated 3 times. By this operation, it was thought that the establised cell line of only a cell with more high infiltration ability was obtained. Using this cell, the cell was injected into the ventriclus sinister of nude mice, and creation of a bone metastasis model was tried. Moreover, it collected, before the cell was set to confluent within the flask, and cell indirect arrival carried out using the thin cell. Consequently, the metastasis to a spine took place to nude mice of three animals among six animals.
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