| Project/Area Number |
15591620
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY |
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Principal Investigator |
HIROTA Koki Toyama Medical and Pharmaceutical University, Department of Anesthesiology, Faculty of Medicine, Associate Professor, 医学部, 助教授 (30218854)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2003: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | rat / hippocampus / aging / general anesthetics / excitatory synaptic transmission / inhibitory synaptic transmission |
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| Research Abstract |
We compared age-related modifications of the effects of general anesthetics in area CA1 of hippocampal slices at 4 weeks (younger group) and 18 weeks (elder group) of age.
Method. Transverse hippocampal slices (400 μm) were prepared from male Wistar rats. Two sets of bipolar electrodes were placed on Schaffer collateral fibers to elicit population spikes (PS) in area CA1 [Sch], and on alveus hippocampi to activate the inhibitory interneurons for GABAergic modulation [Alv].
Results. Volatile anesthetics (isoflurane, sevoflurane) decreased PS[Sch], while intravenous anesthetics (thiopental, propofol) had a minimal effects on PS[Sch]. The extents of inhibitory effects by volatile anesthetics were similar between younger and elder groups. On the other hand, both volatile and intravenous anesthetics reduced PS[Sch+Alv]. The actions were grater on younger group than on elder group.
Conclusion. We demonstrated the age-dependent modification of general anesthetic. actions on the inhibitory synaptic transmission, but not on the excitatory synaptic transmission, in rat CNS preparations in vitro.
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