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Development of synthetic surfactant and its protective effect on alveolar type II cell

Research Project

Project/Area Number 15591621
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionKanazawa University

Principal Investigator

TASHIRO Katsumi  Kanazawa University, Graduate School of Medicine, Department of Anesthesiology and Intensive Care Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (30242556)

Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2004: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordssurfactant protein / E.coli / alveolar type II cell / surfactant inhibition / ischemia reperfusion injury / inflammation / nicotine amide / alveoli / 肺サーファクタント / SP-C / 二酸化炭素 / サイトカイン / 肺虚血再灌流障害 / 肺組織 / リン脂質 / 活性阻害
Research Abstract

(1)Development of a synthetic surfactant protein. A synthetic peptide mimicking surfactant protein-C (Sp-C) was expressed in E.coli, by using pET80 vector and synthetic DNA. However, the yield of synthetic peptide was extremely low. Next, we tried to express the synthetic peptide as fusion proteins fused with DHPP protein using pQE40 vector and with tluoredoxin using pET32a. However, the yields were low in both cases. The low yield is thought to be because of high toxicity of the synthetic peptide to E.coli. So, we could not succeed in expressing synthetic peptide.
(2)Protective of effect of hypercapnia. High concentration of carbon dioxide (suppressed the increase of TNF released from alveolar type II cell stimulated by IL-1 under cell culture. The levels of mRNA for inflammatory cytokine were not elevated. So, high concentration of carbon dioxide inhibits TNF release at the post-transcription level.
(3)Effect of nicotine amide of lung ischemia rperfuusion injury. The left lung of adult rats was reperfirsed after 90 min of ischemia. The arterial oxygen pressure of rats given nicotinamide was 290±80 (SD) mmHg and was significantly higher than that of the control rat (about 100 mmHg). Nicotine amide significantly reduced the lung ischemia reperfusion injury.
(4)Analysis of the lung expansion after surfactant impairment. Mechanical ventilation of immature newborn rabbits treated with surfactant mixed with serum causes the collapse of small alveoli and the overexpansion of large alveoli. However, this surfactant increased the tidal volume to the same extent as normal surfactant. Normal tidal volume dose not necessarily mean normal surfactant function and may lead to overlooking impaired surfactant function.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (16 results)

All 2004 2003 Other

All Journal Article (11 results) Publications (5 results)

  • [Journal Article] 血清で希釈されたサーファクタントによる肺胞の過伸展と虚脱2004

    • Author(s)
      小林勉, 加畑千春, 田代勝己 他
    • Journal Title

      日本界面医学会雑誌 25・1

      Pages: 17-23

    • NAID

      10013598300

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Lung volumes and alveolar expansion pattern in immature rabbits treated with serum-diluted surfactant.2004

    • Author(s)
      Xu Y, Kobayashi T, et al.
    • Journal Title

      Journal of Applied Physiology 97(4)

      Pages: 1408-1413

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Over-distension and collapse of alveoli induced by serum-diluted surfactant (in Japanese)2004

    • Author(s)
      Kobayashi T, Kabata C, Tashiro K, et al.
    • Journal Title

      Journal of Japanese Medical Society for Biological Interface 35

      Pages: 17-23

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Lung volumes and alveolar expansion pattern in immature rabbits treated with serum-diluted surfactant.2004

    • Author(s)
      Xu Y, Kobayashi T, et al.
    • Journal Title

      Journal of Applied Physiology. 97(4)

      Pages: 1408-1413

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Lung volumes and alveolar expansion pattern in immature rabbits treated with serum-diluted surfactant2004

    • Author(s)
      Xu Y, Kobayashi T, et al.
    • Journal Title

      Journal of Applied Physiology 97(4)

      Pages: 1408-1413

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Aerosolized surfactant and dextran for experimental acute respiratory distress syndrome caused by acidified milk in rats2003

    • Author(s)
      Cui XG, Tashiro K, et al.
    • Journal Title

      Acta Anaesthesiologica Scandinavica 47(7)

      Pages: 853-860

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Modified protocols for surfactant therapy in experimental meconium aspiration syndrome2003

    • Author(s)
      Tashiro K, Cui XG, et al.
    • Journal Title

      Biology of the Neonate 83(1)

      Pages: 49-56

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Effects of various forms of surfactant protein C on tidal volume in ventilated immature newborn rabbits2003

    • Author(s)
      Tashiro K, Ohta K, et al.
    • Journal Title

      Journal of Applied Physiology 94(4

      Pages: 1519-1526

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] デキストランは合成サーファクタントの活性を改善する2003

    • Author(s)
      田代勝己, 崔暁光, 紺崎友晴, 他
    • Journal Title

      日本界面医学会雑誌 24・1

      Pages: 71-73

    • NAID

      10013422715

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Effects of various forms of surfactant protein C on tidal volume in ventilated immature newborn rabbits2003

    • Author(s)
      Tashiro K, Ohta K, et al.
    • Journal Title

      Journal of Applied Physiology 94(4)

      Pages: 1519-1526

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Dextran improves function of syntheteic surfactant (in Japanese)2003

    • Author(s)
      Tashiro K, Cui XG, Konzaki T et al.
    • Journal Title

      Journal of Japanese Medical Society for Biological Interface 34

      Pages: 71-73

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Publications] Tashiro K: "Effects of various forms of surfactant protein C on tidal volume in ventilated immature newborn rabbits"Journal of Applied Physiology. 94・4. 1519-1526 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Tashiro K: "Modified protocols for surfactant therapy in experimental meconium aspiration syndrome."Biology of the Neonate. 83・1. 49-56 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Cui XG: "Aerosolized surfactant and dextran for experimental acute respiratory distress syndrome caused by acidified milk in rats."Acta Anaesthesiologica Scandinavica. 477. 853-860 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 田代勝己: "デキストランは合成サーファクタントの活性を改善する"日本界面医学会雑誌. 34・1・2. 71-73 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Xu Y: "Lung volumes and alveolar expansion pattern in immature rabbits treated with serum-diluted surfactant"Journal of Applied Physiology. (印刷中). (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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