| Project/Area Number |
15591638
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Nagasaki University Hospital of Medicine and Dentistry |
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Principal Investigator |
SHIBATA Osamu Nagasaki University Hospital of Medicine and Dentistry, Anesthesiology, Associate Professor, 医学部・歯学部附属病院, 助教授 (80136671)
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| Co-Investigator(Kenkyū-buntansha) |
MAKITA Tetsuji Nagasaki University Hospital of Medicine and Dentistry, Anesthesiology, Instructor, 医学部・歯学部附属病院, 講師 (00229337)
YAMAGUCHI Masakazu Nagasaki University Hospital of Medicine and Dentistry, Anesthesiology, Assistant Professor, 医学部・歯学部附属病院, 助手 (80363498)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2005: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2004: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Anticholinesterases / Rho-kinase / Phosphatidylinositol / Muscarinic M3 receptor / Rat / 抗コリンエステラーゼ / Y-27632 |
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| Research Abstract |
This study was carried out to determine the effects of Rho-kinase inhibitors, Y-27632 and fasudil, on the anticholinesterase (anti-ChE)-induced contractile and phosphatidylinositol responses of rat trachea. In vitro measurements of isometric tension and [^3H] inositol monophosphate (IP_1) formed were conducted by using rat tracheal rings or slices. Neostigmine- and pyridostigmine-induced contractions were almost completely inhibited by Y-27632 and fasudil at 30 μM for each, while acetylcholine-induced contraction was inhibited incompletely, i.e., by 56% by Y-27632 and by 51% by fasudil, at 100 μM for each, respectively. The inhibitory effects of fasudil on neostigmine- and acetylcholine-induced contractions were completely reversed by calyculin-A, a myosin phosphatase inhibitor. Neostigmine-induced IP1 accumulation was attenuated by fasudil at 100 μM. The results suggest that anti-ChEs cause airway smooth muscle contraction in part through activation of the Rho-kinase pathway.
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