• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Effects of local anesthetics and tetrodotoxin on Cultured Neuron of Lymnaea stagnalis

Research Project

Project/Area Number 15591643
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionFaculty of Medicine, University of Miyazaki (2004)
宮崎医科大学 (2003)

Principal Investigator

KASABA Toshiharu  University of Miyazaki, Faculty of Medicine Department of Anesthesiology, Associate Professor, 医学部, 助教授 (80145599)

Co-Investigator(Kenkyū-buntansha) NARUO Hiroaki  Faculty of Medicine, University of Miyazaki, Department of Anesthesiology, Instructor, 医学部, 助手 (10274797)
HAMAKAWA Toshiro  Faculty of Medicine, University of Miyazaki, Department of Anesthesiology, Assistant Professor (50253836)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsLymnaea stagnalis / local anesthetics / lidocaine / tetrodotoxin / neurotoxicty / 水棲かたつむり / 培養細胞 / 細胞内ナトリウム
Research Abstract

To know whether the increase in intracellular Ca^<2+> concentration by lidocaine produces neurotoxicity, we compared morphological changes and Ca^<2+> concentration using fura-2 imaging in the culture neuron of Lymnaea stagnalis.
We used BAPTA-AM, a Ca^<2+> chelator, to prevent the increase of the intracellular Ca^<2+> concentration and Calcimycin A23187,a Ca^<2+> ionophore, to identify the relationship between increased intracellular Ca^<2+> concentration and neuronal damage without lidocaine. Morphological changes were confirmed using trypan blue to stain the cells.
Increasing the dose of lidocaine increased the intracellular Ca^<2+> concentration ; however, there was no morphological damage to the cells in lidocaine at 3x10^<-3> M. Lidocaine at 3x10^<-2> M increased the intracellular Ca^<2+> concentration in both the saline (from 238±63 to 1038±156 nM) and the Ca^<2+>-free medium (from 211±97 to 1046±169 nM) and produced morphological damage and shrinkage with a rugged surface. By the addition of BAPTA-AM, lidocaine at 3x10^<-2> M moderately increased the intracellular Ca^<2+> concentration (from 150±97 to 428±246 nM) and produced morphological damage. These morphologically changed cells were stained to dark blue with trypan blue dye. Ca^<2+> ionophore resulted in an extreme increase of the intracellular Ca^<2+> concentration ; however, no morphological damage was observed.
These results indicated that the morphological damage was induced by lidocaine itself rather than by the increase in the intracellular Ca^<2+> concentration. Controlling the increase in the intracellular Ca^<2+> concentration is not enough to prevent the neurotoxicity of lidocaine.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report

URL: 

Published: 2003-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi