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Mechanisms of postoperative pain in neonate : analysis using in vivo patch clamp recording from superficial dorsal horn neurons.

Research Project

Project/Area Number 15591646
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionSapporo Medical University

Principal Investigator

KAWAMATA Mikito  Sapporo Medical University, School of Medicine, Assistant Professor, 医学部, 講師 (90315523)

Co-Investigator(Kenkyū-buntansha) KANAYA Noriaki  Sapporo Medical University, School of Medicine, Assistant Professor, 医学部, 講師 (10244344)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsSpinal dorsal horn / neonate / incision iniur / in vivo patch-clamp recording
Research Abstract

Background: The aim of the present study was to determine mechanisms of postoperative pain in neonates compared to those in adults, using the incision injury model in rats.
Methods: A single activity of spinal dorsal horn wide-dyanamic-range (WDR) and high-threshold (HT) neurons, that had receptive fields (RFs) on the plantar surface of the hindpaw, was isolated from neonatal rats (postnatal days 1-14) and adult rats (8-10 weeks old) under urethane anesthesis. A 2-5 mm-long incision and 1-cm-long incision were made for neonates and for adults, respectively, in the center of the RF through the skin, fascia and muscle. Neuronal activity (spontaneous activity, RF size and responses to non-noxious and noxious stimuli applied on the center of the RF) was assessed before and 1 hr after the incision had been made. In some WDR neurons in neonate and adult rats, after the incision had been made, receptor antagonists, AP5 (50 mM) and MK-801(50 mM), and a non-NMDA receptor antagonist, CNQX (10 mM) … More , were applied into the surface of the spinal cord.
Results: Responses of WDR neurons to von Frey filament stimuli (4 g and 15 g) were significantly greater in the neonatal rats than those in the adult rats. There were no significant difference in responses of WDR neurons to non-noxious or noxious stimuli between P1-P7 neonatal rats and P8-P14 neonatal rats. WDR neurons in neonatal rats often showed prolonged after-discharge following brief noxious stimuli. Subsequent spontaneous activity of WDR following incision lasted for a longer period of time in neonate rats than in adult rats after the incision had been made. Responses to non-noxious and noxious stimuli of WDR neurons significantly increased in the neonatal rats and adult rats after the incision (P < 0.01), compared to the pre-incision values. Eighty percent of HT neurons in neonatal rats began to respond to non-noxious stimuli after the incision had been made, whereas HT neurons could not respond to non-noxious stimuli in the adult rats after the incision. When AP5 and MK-801 were applied onto the spinal cord in neonatal WDR neurons 1 hr after the incision had been made, the increased responses to non-noxious and noxious stimuli almost completely abolished. However, AP5 and MK-801 did not significantly reduce the increased responses of WDR neurons to non-noxious or noxious stimuli in adult rats after the incision had been made. Application of CNQX also abolished the responses of WDR neurons to mechanical stimuli after the incision had been made. Conclusions : These findings suggest the mechanisms of postoperative pain in neonates are different from those in adults, possibly depending on the different excitatory synapse transmission through NMDA receptors in the spinal cord. Less

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (19 results)

All 2005 2004 Other

All Journal Article (14 results) Publications (5 results)

  • [Journal Article] Sepsis stage dependently and differentially attenuates the effects of non-depolarizing neuromuscular blockers on the rat diaphragm in vitro.2005

    • Author(s)
      Narimatsu E, Niiya T, Kawamata M, Namiki A.
    • Journal Title

      Anesthesia and Analgesia 100

      Pages: 823-829

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Effects of halothane and isoflurane on hyperexcitability of spinal dorsal horn neurons after incision in the rat.2005

    • Author(s)
      Kawamata M, Narimatsu E, Kozuka Y, Takahashi T, Sugino S, Niiya T, Namiki A.
    • Journal Title

      Anesthesiology 102

      Pages: 165-174

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Changes in properties of spinal dorsal horn neurons and their sensitivity to morphine after spinal cord injury in the rat.2005

    • Author(s)
      Wang J, Kawamata M, Namiki A
    • Journal Title

      Anesthesiology 102

      Pages: 152-164

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Changes in response properties and receptive fields of spinal dorsal horn neurons in rats after surgical incision in hairy skin.2005

    • Author(s)
      Kawamata M, Koshizaki M, Shimada SG, et al.
    • Journal Title

      Anesthesiology 102

      Pages: 141-151

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Sepsis stage dependently and differentially attenuates the effects of non-depolarizing neuromuscular blockers on the rat diaphragm in vitro2005

    • Author(s)
      Narimatsu E, Niiya T, Kawamata M, Namiki A.
    • Journal Title

      Anesthesia and Analgesia 100

      Pages: 823-829

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] A Effects of halothane and isoflurane on hyperexcitability of spinal dorsal horn neurons after incision in the rat2005

    • Author(s)
      Kawamata M, Narimatsu E, Kozuka Y, Takahashi T, Sugino S, Niiya T, Namiki
    • Journal Title

      Anesthesiology 102

      Pages: 165-74

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Changes in propertie s of spinal dorsal horn neurons and their sensitivity to morphine after spinal cord injury in the rat2005

    • Author(s)
      Wang J, Kawamata M, Namiki A
    • Journal Title

      Anesthesiology 102

      Pages: 152-164

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Changes in response properties and receptive fields of spinal dorsal horn neurons in rats after surgical incision in hairy skin2005

    • Author(s)
      Kawamata M, Koshizaki M, Shimada SG; et al.
    • Journal Title

      Anesthesiology 102

      Pages: 141-151

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Sepsis stage dependently and differentially attenuates the effects of non-depolarizing neuromuscular blockers on the rat diaphragm in vitro.2005

    • Author(s)
      Narimatsu E, Niiya T, Kawamata M, Namiki A
    • Journal Title

      Anesthesia and Analgesia 100

      Pages: 823-829

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Changes in properties of spinal dorsal horn neurons and their sensitivity to morphine after spinal cord injury in the rat.2005

    • Author(s)
      Wang J, Kawamata M, Namiki A.
    • Journal Title

      Anesthesiology 102

      Pages: 152-164

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Effects of pretreatment with spinal MK-801,CNQX and tetrodotoxin on development of hyperexcitability of spinal dorsal horn neurons after incision in the rat2005

    • Author(s)
      Kawamata M, Narimatsu E, Kanaya N, Nishikawa K, Namiki A
    • Journal Title

      Anesthesia and Analgesia (In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Left ventricular mechanical performance in elderly patients after induction of anaesthesia.2004

    • Author(s)
      Nishikawa K, Kanaya N, Kawamata M, Namiki A.
    • Journal Title

      Anaesthesia 59

      Pages: 948-953

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Left ventricular mechanical performance in elderly patients after induction of anaesthesia2004

    • Author(s)
      Nishikawa K, Kanaya N, Kawamata M, Namiki A.
    • Journal Title

      Anaesthesia 59

      Pages: 948-953

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Left ventricular mechanical performance in elderly patients after induction of anaesthesia.2004

    • Author(s)
      Nishikawa K, Kanaya N, Kawamata M, Namiki A
    • Journal Title

      Anaesthesia 59

      Pages: 948-953

    • Related Report
      2004 Annual Research Report
  • [Publications] Narimatsu E, Kawamata M, Hase M, Kurimoto Y, Asai Y, Namiki A.: "Severe paradoxical intracranial embolism and pulmonary emboli during hip hemiarthroplasty"Br J Anaesth. 91(6). 911-913 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Koshizaki M, Kawamata M, Shimada SG, Saito Y, Collins JG.: "5-HT3 receptors partially mediate halothane depression of spinal dorsal horn sensory neurons."Anesth Analg. 96(4). 1027-1031 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Nakayama M, Kanaya N, Edanaga M, Namiki A.: "Hemodynamic and bispectral index responses to tracheal intubation during isoflurane or sevoflurane anesthesia"J Anesth. 17(4). 223-226 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Wang J, Kawamata M, Namiki A: "Physiological and pharmacological changes in properties and receptive fields of spinal dorsal horn neurons after spinal cord injury in the rat"Anesthesiology. In press. (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] 川真田樹人, 並木昭義: "PCA(自己調節鎮痛)の実際"克誠堂. 9 (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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