| Project/Area Number |
15591647
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
NARIMATSU Eichi Sappro Medical University, Depts. of Anesthesiology and Critical Care Medicine, Associate Professor, 医学部, 助教授 (70295343)
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| Co-Investigator(Kenkyū-buntansha) |
IMAIZUMI Hitoshi Sappro Medical University, Depts. of Anesthesiology and Critical Care Medicine, Associate Professor, 医学部, 助教授 (70203304)
KAWAMATA Mikito Sappro Medical University, Dept. of Anesthesiology, Assistant Professor, 医学部, 講師 (90315523)
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| Project Period (FY) |
2003 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2005: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | rocuronium / pancuronium / d-tubocurarine / sepsis / neuromuscular blocker / endplate potential / acetylcholine potential / cecal ligation and puncture / D-tubocurarine / neuromuscular Blocker / quantum content |
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| Research Abstract |
Sepsis is known to attenuate the actions of nondepolarizing neuromuscular blockers clinically. This study was planned to investigate the effect of sepsis on neurommcular transmission and actions of nondepolarizing neuromuscular blockers.
Results
The effect of sepsis on neuromuscular transmission
Late sepsis inc-eased endplate potential amplitude, increased quantal acetylcholine release from the motor nerve terminal, decreased postjunctional acetylcholine sensitivity of endplate membrane and increased electrical excitability of sarcoplasmic membrane.
The effect of sepsis on actions of nondepolarizing neuromuscular blockers
Rocronium, pancuronium and d-tubocurarine dose-dependently decreased twitch tension elicited by nerve stimulation. Early and late sepsis shifted these curves rightwards. The rightward shift was largest in pancuronium and smallest in d-tubocurarine under the conditions of both early and late sepsis. The rightward shift in late sepsis was larger than that in early sepsis for
… More
each neuromuscular blocker.
Late sepsis potentiated the rocuronium-induced decrease of endplate potential amplitude. Late sepsis did not alter the rocuronium-induced decrease of quantal acetylcholine release and the rocuronium-induced decrease of postjunctional acetylcholine sensitivity. Rocuronium did not alter the late sepsis-induced increase in electrical excitability of sarcoplasmic membrane.
These results indicate the following evidences. Sepsis affects on the stages of motor nerve terminal, endplate membrane and sarcoplasmic membrane in neuromuscular transmission and on the actions of nondepolarizing neuromuscular blockers on these stages in various manners. The main and significant mechanism of sepsis inducing the attenuations of the actions of nondepolarizing neuromuscular blockers is the increase in electrical excitability of sarcoplasmic membrane, but does not exist in the quantal acetylcholine release or postjunctional acetylcholine sensitivity. The sepsis-induced attenuations in the actions of nondepolarizing neuromuscular blockers depend on the stage of sepsis and kinds of the neuromuscular blockers. Less
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