| Project/Area Number |
15591666
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Anesthesiology/Resuscitation studies
|
|---|
| Research Institution | KANAZAWA MEDICAL UNIVERSITY |
|---|
Principal Investigator |
TSUCHIDA Hideaki Kanazawa Medical Univ., Anesthesiology, Professor, 医学部, 教授 (20155394)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
UEDA Yoshimichi Kanazawa Medical Univ., Pathology, Professor, 医学部, 教授 (50271375)
SEKI Sumihiko Kanazawa Medical Univ., Anesthesiology, Associate Prof., 医学部, 助教授 (50315503)
HIDAKA Kohji Kanazawa Medical Univ., Anesthesiology, Instructor, 医学部, 助手 (50410337)
柳川 慎平 金沢医科大学, 医学部, 助手 (60329399)
白塚 秀之 金沢医科大学, 医学部, 助手 (00329385)
|
|---|
| Project Period (FY) |
2003 – 2005
|
| Project Status |
Completed (Fiscal Year 2005)
|
| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2005: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥1,100,000 (Direct Cost: ¥1,100,000)
|
|---|
| Keywords | Mongolian Gerbil / Hippocampus / Apoptosis / Heat Shock Protein 90 / 熱ショックタンパク90 |
|---|
| Research Abstract |
Although apoptosis is most likely the main cause of delayed neuronal death (DND) in the hippocampal pyramidal neurons after transient ischemia, the molecular mechanisms underlying this phenomenon have not been elucidated clearly. Recently Heat Shock Protein 90 (HSP90) attracts attention as one of protective factor for stress, but the details of its function are scarsely elucidated.
Male Mongolian gerbils (n=), weighing 60 to 70 g, were anesthetized with isoflurane. Transient forebrain ischemia was induced by occluding the bilateral common carotid arteries for 5 minutes. A sham-operation group was not subjected to ischemia and served as the negative control. The animals were sacrificed 6-120 hours after ischemia and the hippocampi were dissected. We analyzed expression and localization of HSP90 using RT-PCR, Western blot analysis, and immunohistochemistry. In addition, HSP90 was induced by geranylgeranyl acetone and the function of HSP90 was examined.
RT-PCR and Western blot analysis showed that HSP90 mRNA was up-regulated at 6 to 48 hours and HSP90 was increased at 12 to 48 hours after ischemia in the pyramidal neurons. While an increase in HSP90 was also verified by immunohistochemistry in the hippocampus after ischemia, the expression was weak in the CAI region compared to that in CA2. Intraperitoneal administration of geranylgeranylacetone, 400 mg/kg, 4 hours before ischemia induced expression of HSP90 at 48 hours after ischemia and ameliorated DNA fragmentation and DND at 96 hours after ischemia in the pyramidal neurons.
These results strongly indicate that HSP90 is protective against DND after transient ischemia in the Mongolian Gerbil. In addition, difference in the ability of HSP90 induction may be involved in selective neuronal vulnerability.
|
