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Biochemical analysis of bladder detrusor muscle in voiding dysfunction

Research Project

Project/Area Number 15591697
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionNagasaki University Graduate School of Biomedical Sciences

Principal Investigator

NOGUCHI Mitsuru  Nagasaki University Graduate School of Biomedical Sciences, Division of Nephro-Urology, Assistant, 大学院・医歯薬学総合研究科, 助手 (00325648)

Co-Investigator(Kenkyū-buntansha) KANETAKE Hiroshi  Nagasaki University Graduate School of Biomedical Sciences, Division of Nephro-Urology, Professor, 大学院・医歯薬学総合研究科, 教授 (50100839)
NOMATA Koichiro  Nomata Nagasaki University Hospital of Medicine and Dentistry, Division of Nephro-Urology, Lecturer, 医学部・歯学部附属病院, 講師 (80189430)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsVoiding dysfunction / Gap junction / Connexin 43 / Detrusor muscle / 排尿障害
Research Abstract

Gap junctional intercellular communication may play an important role in cell growth, development and differentiation. Gap junctional intercellular channels allow direct movement between neighboring cells of ions, molecules and neurotransmitters less than 1.2 kDa. Therefore, it is considered that the gap junction may play an important role in synchronous detrusor contraction in voiding function. We investigated the alteration of gap junctional protein, connexin-43 (Cx-43) in rat detrusor muscle with voiding dysfunction model. Partial bladder outlet obstruction (BOO) rats and diabetes rats induced by streptozotosin were used for voiding disfunction model. Eight weeks later, cystmetrical investigation, alteration of the Cx-43 expression and extracellular signal-regulated kinase (ERK1/2) in detrusor muscle were analyzed. No detrusor contraction was observed in only BOO rat at 8 weeks. Cx-43 was expressed on cell membranes of detrusor muscle in control and DM rats. However, Cx-43 was revealed internal localization from cell membrane to cytoplasm or nuclei. The expression levels of phosphorylated Cx-43 of detrusor muscle in BOO were higher than those of control and DM rats. Furthermore, ERK1/2 activation of detrusor muscle in only BOO rats. Cx-43 of the detrusor mousle in BOO might be regulated by phosphorylation and ERK 1/2 activation, and the alterations of Cx-43 were considered to cause disruptions of gap junctions. These data suggest that the disruptions of gap junction in detrusor muscle may be one of the causes of voiding dysfunction.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (10 results)

All 2005 Other

All Journal Article (8 results) Publications (2 results)

  • [Journal Article] 膀胱排尿筋におけるギャップ結合の役割2005

    • Author(s)
      野口 満 他
    • Journal Title

      西日本泌尿器科 67.5

      Pages: 219-223

    • NAID

      10017397470

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Decreased cellular membrane expression of gap junctional protein, connexin 43, in rat detrusor muscle with chronic partial bladder outlet obstruction.2005

    • Author(s)
      Mori K, Noguchi M et al.
    • Journal Title

      Urology 65.6

      Pages: 1254-1259

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] 膀胱におけるcell-cell communication2005

    • Author(s)
      野口 満
    • Journal Title

      Urology Today 12.4

      Pages: 24-27

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The role of gap junction in the detrusor muscle2005

    • Author(s)
      Mitsuru Noguchi, Ken-ichi Mori, Shohei Tobu, Manabu Matsuo, Koichiro Nomata, Hiroshi Kanetake
    • Journal Title

      Nishinihon J Urol 67

      Pages: 219-223

    • NAID

      10019219853

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Decreased cellular membrane expression of gap junctional protein, connexin 43, in rat detrusor muscle with chronic partial bladder outlet obstruction.2005

    • Author(s)
      Mori K, Noguchi M, Manabu matsuo, Koichiro Nomatam Hiroshi Kanetake
    • Journal Title

      Urology 65 (6)

      Pages: 1254-1258

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Cell to cell communication in bladder2005

    • Author(s)
      Mitsuru Noguchi
    • Journal Title

      Urology Today 12(4)

      Pages: 24-27

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Decrease Cellular Membrane Expression of Gap Junctional Protein, Connexin-43, in Rat Detrusor Muscle with Chronic Bladder Outlet Obstruction2005

    • Author(s)
      K Mori, M Noguchi, et al.
    • Journal Title

      Urology 65(In press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 膀胱排尿筋におけるギャップ結合の役割2005

    • Author(s)
      野口 満, 他
    • Journal Title

      西日本泌尿器科学会雑誌 67(In press)

    • NAID

      10017397470

    • Related Report
      2004 Annual Research Report
  • [Publications] K.Mori, M.Noguchi, M.Matsuo, K.Nomata, H.Kanetake: "Internalization of gap functional protein, connexin-43 in rat with bladder outlet obstruction"International Continence Society 33^<rd> annual meeting. Proceeding. 14-15 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] M.Matsuo, M.Noguchi, K.Mori, K.Nomata, H.Kanetake: "Immunohistochemical analysis of gap functional protein, connexin-43 in rat detrusor muscle with streptozotocin-induced diabetes"International Continence Society 33^<rd> annual meeting. Proceeding. 453-454 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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