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A research for the involvement of renin-angiotensin system in placental formation

Research Project

Project/Area Number 15591740
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionSaitama Medical School (2005)
Nagoya University (2003-2004)

Principal Investigator

ITAKURA Atsuo  Saitama Medical School, Professor, 医学部, 教授 (70262897)

Co-Investigator(Kenkyū-buntansha) OKADA Mayumi  Nagoya University, Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (90324433)
水谷 栄彦  名古屋大学, 大学院・医学系研究科, 教授 (00159162)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2005: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsangitensin II / trophoblast / extravillous trophoblast / choriocarcinoma / pregnancy induced hypertension / cytotrophoblast / 妊娠中毒症
Research Abstract

Angiotensin II (Ang II), a bioactive peptide active in the renin-angiotensin system (RAS), has various biological functions besides its role as a potent vasoconstrictor. It has been shown to inhibit human extravillous trophoblast migration via PAI-1 activation through the Ang II type 1 receptor (AT1R). But, our previous study revealed induction of choriocarcinoma cell proliferation. The data suggested an involvement of the local RAS in tumor growth, contrasting with suppression of extravillous trophoblast invasion by RAS activation. In the present study we therefore investigated the effects of Ang II on choriocarcinoma cell migration in vitro using Boyden chamber and invasion assays. Ang II (10^<-8>M) promoted migration and invasion by a choriocarcinoma cell line (BeWo) and augmented random cell mobility on checker board analysis. Immunoblotting showed that Ang II activated the phosphorylation of FAK,ERK 1/2, and Akt in BeWo cells. Furthermore the Ang II effects on cell migration and invasion were abolished by a selective AT1R antagonist and an Akt inhibitor. The present results suggest that Ang II induced migration and invasion of choriocarcinoma cell probably involves PI3K/Akt following binding to the AT1R.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report
  • Research Products

    (20 results)

All 2006 2005 Other

All Journal Article (17 results) Publications (3 results)

  • [Journal Article] Angiotensin II augments migration and invasion of choriocarcinoma cells involves PI3K activation through the AT1 receptor.2006

    • Author(s)
      S.Ishimatsu, A.Itakura, et al.
    • Journal Title

      Placenta (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Methods for myometrium closure and other factors impacting effects on cesarean section scars of the uterine segment detected by the ultrasonography2006

    • Author(s)
      H.Hayakawa, A.Itakura, et al.
    • Journal Title

      Acta Obstet Gynecol Scand (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] A case of placenta accreta complicated by severe IUGR and maternal coagulopathy2006

    • Author(s)
      M.Okada, Itakura, et al.
    • Journal Title

      Acta Obstet Gynecol Scand (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A triplet pregnancy featuring a 47, XY+9 heterozygous complete mole coexisting with two fetuses at 9 weeks2006

    • Author(s)
      M.Suzuki, A.Itakura, et al.
    • Journal Title

      Acta Obstet Gynecol Scand (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Successful treatment of a heterotopic cervical pregnancy ant twin gestation by sonographically guided instillation of hyperosmolar glucose2006

    • Author(s)
      M.Suzuki, A.Itakura, et al.
    • Journal Title

      Acta Obstet Gynecol Scand (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Angiotensin II augments migration and invasion of choriocarcinoma cells involves PI3K activation through the AT1 receptor.2006

    • Author(s)
      S.Ishimatsu, A.Itakura, et al.
    • Journal Title

      Placenta 2006 (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Methods for myometrium closure and other factors impacting effects on cesarean section scars of the uterine segment detected by the ultrasonography2006

    • Author(s)
      H.Hayakawa, A.Itakura, et al.
    • Journal Title

      Acta Obstet Gynecol Scand 2006 (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A case of placenta accreta complicated by severe IUGR and maternal coagulopathy2006

    • Author(s)
      H.Okada, A.Itakura, et al.
    • Journal Title

      Acta Obstet Gynecol Scand 2006 (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] A triplet pregnancy featuring a 47, XY+9 heterozygous complete mole coexisting with two fetuses at 9 weeks2006

    • Author(s)
      M.Suzuki, A.Itakura, et al.
    • Journal Title

      Acta Obstet Gynecol Scand 2006 (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Successful treatment of a heterotopic vical pregnancy and twin gestation by sonographically guided illation of hyperosmolar glucose2006

    • Author(s)
      Suzuki, A.Itakura, et al.
    • Journal Title

      Acta Obstet Gynecol Scand 2006 (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Angiotensin II augments migration and invasion of choriocarcinoma cells involves P13K activation through the AT1 receptor.2006

    • Author(s)
      S.Ishimatsu, A.Itakura, et al.
    • Journal Title

      Placenta (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A case of placenta accreta complicated by severe IUGR and maternal coagulopathy.2006

    • Author(s)
      M.Okada, A.Itakura, et al.
    • Journal Title

      Acta Obstet Gynecol Scand (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Successful treatment of a heterotopic cervical pregnancy and twin gestation by sonographically guided instillation of hyperosmolar glucose2006

    • Author(s)
      M.Suzuki, A.Itakura, et al.
    • Journal Title

      Acta Obstet Gynecol Scand (in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Placental leucine aminopeptidase might regulate the effects of oxytocin with resolution in endothelial cells.2005

    • Author(s)
      M.Sano, A.Itakura, et al.
    • Journal Title

      Med Sci Monit 11

      Pages: 195-199

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Annual Research Report 2005 Final Research Report Summary
  • [Journal Article] Possible antenatal and perinatal related factors in development of cystic periventricular leukomalacia2005

    • Author(s)
      Y.Murata, A.Itakura et al.
    • Journal Title

      Brain Dev 27

      Pages: 17-21

    • NAID

      10015452887

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Involvement of placental peptidases associated with renin-angiotensin systems in preeclampsia2005

    • Author(s)
      A.Itakura, S.Mizutani
    • Journal Title

      BBA Proteins and proteomics (掲載予定)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Changes in placental dipeptidyl peptidase IV in pre-eclampsia with intrauterine growth restriction.2005

    • Author(s)
      M.Nishikawa, A.Itakura, et al.
    • Journal Title

      Horm Metab Res (掲載予定)

    • Related Report
      2004 Annual Research Report
  • [Publications] Y.Tsukahara, A.Itakura et al.: "Umbilical plasma kininase I activity in fetal hypoxia"Hormone & Metabolic Research. 35. 583-587 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] S.Matsuura, A.Itakura et al.: "Effects of estradiol administration on feto-placental growth in rat"Early Human Development. (in press). (2004)

    • Related Report
      2003 Annual Research Report
  • [Publications] C.Ito, A.Itakura, et al.: "A prospective evaluation of the effects of salpingectomy on endometria 1 lymphocyte clusters in cases with hydrosalpinges"Fertility and Sterility. (in press). (2004)

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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