| Project/Area Number |
15591751
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
SAMURA Osamu Hiroshima University, Hospital, Assistant, 病院, 助手 (90314757)
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| Co-Investigator(Kenkyū-buntansha) |
MIHARU Norio Hiroshima University, Hospital, Assistant Professor, 病院・講師 (30253082)
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| Project Period (FY) |
2003 – 2005
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| Project Status |
Completed (Fiscal Year 2005)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 2005: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | FISH / Real Time PCR / Microchimerism / Fetal DNA in maternal blood / SRY / Microshimerism / Real time PCR法 |
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| Research Abstract |
Microchimerism is the state that low-level donor cells or progenitor cells maintain in a host. Recently, microchimerism has been considered as the possible pathogenesis of some diseases, including auto immune diseases. Our aim was to examine whether or not cell-free fetal DNA existed in the serum collected from non-pregnant women who were suffering from thyroid disease. After obtaining written informed consent, we took peripheral blood samples from 99 non-pregnant women suffering from thyroid disease. For the control group, we recruited 71 non-pregnant women without either thyroid disease or auto immune diseases. DNA was extracted from the serum. Then, we performed a real-time quantitative polymerase chain reaction (PCR) using the SRY sequence as a marker of fetal microchimerism. We detected the SRY sequence in 6 of 99 patients (6.1%). The longest period after the birth of the youngest son was 56 years. On the other hand, the SRY sequence was not detectable in the control group (0%). This is the first report to examine microchimerism in the serum from patients with thyroid disease. We detected cell-free fetal DNA in serum specimens taken from non-pregnant women suffering from thyroid disease. Our findings suggest that fetal microchimerism is a possible pathogenesis of thyroid diseases.
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