| Project/Area Number |
15591766
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Oita University (2004-2006)
大分医科大学 (2003) |
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Principal Investigator |
KAWANO Yasushi Oita Univ., Dept. of Obstet. & Gynecol., Assist Professor, 医学部, 講師 (40274758)
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| Co-Investigator(Kenkyū-buntansha) |
NARAHARA Hisashi Oita Univ., Dept. of Obstet. & Gynecol., Professor, 医学部, 教授 (60211447)
FUKUDA Junichiro Oita Univ., Dept. of Obstet. & Gynecol., Instructor, 医学部, 助手 (20381048)
宮川 勇生 大分大学, 医学部, 教授 (70040607)
嶺 真一郎 大分大学, 医学部, 助手 (00336273)
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| Project Period (FY) |
2003 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2005: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2004: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2003: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Vascular endothelial growth factor / Endometrial stromal cells / Follicular fluid / 顆粒膜細胞 / 子宮内膜 / 血管新生 |
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| Research Abstract |
Our objective was to clarify the physiological role of angiogenic factor or anti-angiogenic factor by ovarian granulosa cell, endometrial stromal cells (ESC) and amnion-derived (WISH) cells.
Several angiogenic factors were present in human follicular fluid. macrophage colony-stimulating factor, monocyte chemoattractait protein-1 and macrophage inflammatory protein-3 a were secreted by granulosa cells. These factors were up-regulared by interleukin-1 a and tumor necrosis factor-a by these cells.
We reported that VEGF was produced by ESC. In adition thrombospondin (TSP)-1 production was increased by interferon (IFN)-γ and was decreased by epidermal growth factor (EGF) in a dose-dependent manner. TSP-1 production and TSP-1 mRNA expression were also increased by medroxyprogesterone (MPA) and cyclic AMP during in vitro decidualization..
WISH cells were cultured, and VEGF production was increased by EGF or insulin-like growth factor (IGF)-1 in a dose-dependent manner. The activation of MAP kinase and akt, which is phosphorylated by PI 3-kinase, were detected by western blot analysis. VEGF production was significantly decreased following pretreatment with MEK inhibitor (U0126) or PI 3-kinase inhibitor (wortmannin), and then treated with EGF or IGF-1.
It is suggest ed that angiogenic factor may contribute to the neovascularization and proliferation of the ovary, uterine endometrium, and placenta.
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