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Ultrastructural enzymehistochemical and cell-biological analysis of the preterm dilivery -relationship between fetal cells and preterm delivery-

Research Project

Project/Area Number 15591775
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionJICHI MEDICAL SCHOOL

Principal Investigator

MATSUBARA Shigeki  Jichi Medical School, Medicine, Professor, 医学部, 教授 (20209597)

Co-Investigator(Kenkyū-buntansha) WATANABE Takashi  Jichi Medical School, Medicine, Associate Professor, 医学部, 助教授 (90265265)
OKUCHI Akihide  Jichi Medical School, Medicine, Assistant Professor, 医学部, 講師 (10306136)
Project Period (FY) 2003 – 2004
Project Status Completed (Fiscal Year 2004)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥800,000 (Direct Cost: ¥800,000)
Keywordsamnion / chorion / placenta / macrophage / enzymehistochemistry / preterm delivery / leukocyte / phagocytosis / 組織化学
Research Abstract

1.We characterized the morphology of the human amniotic epithelial cells in term pregnancies. In the chemically fixed amnion, there were two types of cells with different degree of cytoplasmic darkness. However, there were no differences in the number and the shape of Golgi apparatus and mitochondria between the cells. The distribution pattern and the intensity of the enzyme detected by the enzymehistochemistry did not differ between cells, either. Freeze-substitution fixation abolished the differences of the cytoplasmic darkness between cells. We concluded that human amniotic epithelial cells consisted of the homogeneous cell population as far as the morphological features were concerned.
2.In the chorioamnion from women with preterm delivery there were abundant polymorphonuclear leukocytes. These leukocytes exhibited morphological features characteristic of phagocytosing leukocytes, ie. : appearance of phagocytotic vacuole with myeloperoxidase on the phagocytotic membrane. These activated leukocytes accumulated just in front of the basement membrane of the chorioamnion. We concluded that in the chorioamnion of the preterm delivery maternal leukocytes accumulated at the basement membrane of this tissue and they phagocytosed various cell debris in this membrane. The phenomenon observed here may have close association with the development of the preterm delivery.
3.We developed dual staining for acid phosphatase and glucose-6-phosphate dehydrogenase histochemistry at an ultrastructural level. We applied this technique to the fetal placental macrophages in preterm delivery. The macrophages with positive staining for glucose-6-phosphate dehydrogenase were defined as activated macrophages. The number of activated fetal placental macrophages were significantly larger in the placenta with preterm delivery than that with normal term delivery. We concluded that fetal placental macrophages had close association with the initiation of the preterm delivery.

Report

(3 results)
  • 2004 Annual Research Report   Final Research Report Summary
  • 2003 Annual Research Report
  • Research Products

    (16 results)

All 2004 2003 Other

All Journal Article (15 results) Publications (1 results)

  • [Journal Article] Golgi apparatus and mitochondria of human amniotic epithelial cells.2004

    • Author(s)
      Matsubara S, Iwasaki R
    • Journal Title

      Acta Histochemica et Cytochemica 37

      Pages: 407-409

    • NAID

      110003161221

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The risk of birth defects in dichorionic twins conceived by assisted reproductive technology.2004

    • Author(s)
      Kuwata T, Matsubara S, Ohkuchi A, Watanabe T, Suzuki M et al.
    • Journal Title

      Twin Research 7

      Pages: 223-227

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Changes in activity of plasma thrombin activatable fibrinolysis inhibitor in pregnancey.2004

    • Author(s)
      Watanabe T, Minakami H, Sakata Y, Matsubara S, Sato I, Suzuki M
    • Journal Title

      Gynecologic and Obstetric Investigation 58

      Pages: 19-21

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Golgi apparatus and mitochondria of human amniotic epithelial cells.2004

    • Author(s)
      Matsubara S et al.
    • Journal Title

      Acta Histochem Cytochem 37

      Pages: 407-409

    • NAID

      110003161221

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] The risk of birth defects in dichorionic twins conceived by assisted reproductive technology.2004

    • Author(s)
      Kuwata T, et al.
    • Journal Title

      Twin Research 7

      Pages: 223-227

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Changes in activity of plasma thrombin activatable fibrinolysis inhibitor in pregnancy.2004

    • Author(s)
      Watanabe T, et al.
    • Journal Title

      Gynecol Obstet Invest 58

      Pages: 19-21

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Golgi apparatus and mitochondria of human amniotic epithelial cells.2004

    • Author(s)
      Matsubara S, Iwasaki R
    • Journal Title

      Acta Histochemica et Cytochemica 37(6)

      Pages: 407-409

    • NAID

      110003161221

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Changes in activity of plasma thrombin activatable fibrinolysis inhibitor in pregnancy.2004

    • Author(s)
      Watanabe T, Minakami H, Sakata Y, Matsubara S, Sato I, Suzuki M
    • Journal Title

      Gynecologic and Obstetric Investigation 58

      Pages: 19-21

    • Related Report
      2004 Annual Research Report
  • [Journal Article] Hofbauer cell activation and its increased glucose-6-phosphate dehydrogenase activity in second-trimester spontaneous abortion.2003

    • Author(s)
      Matsubara S, Takayama T, Watanabe T, Ohkuchi A, Suzuki et al.
    • Journal Title

      American Journal of Reproductive Immunology 49

      Pages: 202-209

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Human amniotic epithelial cells are morphologically homogeneous : enzymehistochemical, tracer, and freeze-substitution fixation study.2003

    • Author(s)
      Iwasaki R, Matsubara S, Takizawa T, Takayama T, Yashiro T, Suzuki M
    • Journal Title

      European Journal of Histochemistry 47

      Pages: 223-232

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Chorioamnionitis and polymorphonuclear leukocytes : morphology of polymorphonuclear leukocytes infiltrating the fetal membranes.2003

    • Author(s)
      Takayama T, Matsubara S, Watanabe T, Suzuki M et al.
    • Journal Title

      Placenta 24

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2004 Annual Research Report 2004 Final Research Report Summary
  • [Journal Article] Hofbauer cell activation and its increased glucose-6-phosphate dehydrogenase activity in second trimester-spontaneous abortion : An ultrastructural dual staining enzyme-cytochemical study.2003

    • Author(s)
      Matsubara S, et al.
    • Journal Title

      Am J Reprod Immunol 49

      Pages: 202-209

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Human amniotic epithelial cells are morphologically homogeneous : enzymehistochemical, tracer, and freeze-substitution fixation study.2003

    • Author(s)
      Iwasaki R, et al.
    • Journal Title

      Eur J Histochem 47

      Pages: 223-232

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Chorioamnionitis and polymorphonuclear leukocytes : morphology of polymorphonuclear leukocytes infiltrating the fetal membranes.2003

    • Author(s)
      Takayama T, et al.
    • Journal Title

      Placenta 24

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2004 Final Research Report Summary
  • [Journal Article] Hofbauer cell activation and its increased glucose-6-phosphate dehydrogenase activity in second-trimester spontaneous abortion.2003

    • Author(s)
      Matsubara S, Takayama T, Watanabe T, Ohkuchi A, Suzuki M et al.
    • Journal Title

      American Journal of Reproductive Immunology 49

      Pages: 202-209

    • Related Report
      2004 Annual Research Report
  • [Publications] Matsubara S, Iwasaki R ら: "Human amniotic epithelial cells are morphologically homogeneous."European Journal of Histochemistry. (in press).

    • Related Report
      2003 Annual Research Report

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Published: 2003-04-01   Modified: 2016-04-21  

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