| Project/Area Number |
15591795
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Yamagata University School of Medicine |
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Principal Investigator |
KOIKE Shuji Yamagata University School of Medicine, Department of Othorhinolaryngology, head and neck surgery, 医学部, 講師 (10205297)
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| Co-Investigator(Kenkyū-buntansha) |
GOTO Kaoru Yamagata University School of Medicine, Department of Anaatomy and cell biology, 医学部, 教授 (30234975)
ITO Tsukasa Yamagata University School of Medicine, Department of Othorhinolaryngology, head and neck surgery, 医学部, 助手 (50344809)
HOZUMI Yasukazu Yamagata University School of Medicine, Department of Anaatomy and cell biology, 医学部, 助手 (00372334)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | diacylglycerol / protein kinase C / diacylglycerol kinase / human malignant tumor cells / RT-PCR / specific antibody / DGK inhibitor / cell growth / 扁平上皮癌 / 甲状腺癌 / 扇平上皮癌 |
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| Research Abstract |
Diacylglycerol (DG) is one of the important second messengers, which serves as an activator of protein kinase C (PKC). DG kinase (DGK) phosphorylates DG to generate phosphatidic acid, thus DGK is considered to be a regulator of PKC activity through attenuation of DG. Recent studies have revealed molecular structures of several DGK isozymes from mammalian species, and showed that most of the isozymes are expressed ih the brain in various amounts. Five DGK isozyme cDNA have been cloned from rat brain library (DGKα, -β, -γ, -ζ, -ι) in Department of Anatomy and cell biology, Yamagata University School of medicine. mRNA expressions of DGK isozymes in rat brain have been studied by in situ hybridization histochemistry, but their mRNA expressions in human malignant tumor cells, especially epithelial cells, have not been examined. In this study, we examined the mRNA expression of DGK isozymes in human squamous cell carcinoma cell lines. To study the localization and functional role of DGKζ, we generated the specific antibody against rat DGKζ and examined the specificity of the antibody. Furthermore, human malignant tumor cell has been cultured with DGK inhibitors and it was revealed that DGK inhibitor might suppress cell growth. It shows that DGK may have some relations to the molecular mechanism of cell growth.
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