| Project/Area Number |
15591809
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
FUJINO Kiyohiro KYOTO UNIVERSITY, Graduate School of medicine, Assistant Professor, 医学研究科, 助手 (50359832)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAGAWA Takayuki KYOTO UNIVERSITY, Graduate School of medicine, Assistant Professor, 医学研究科, 助手 (50335270)
TSUJI Jun KYOTO UNIVERSITY, Graduate School of medicine, Lecturer, 医学研究科, 講師 (30252448)
KITA Tomoko KYOTO UNIVERSITY, Graduate School of medicine, Researcher, 医学研究科, 研究員(科学技術振興) (20362519)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2004: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2003: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | Notch signaling / RBP-J / Cre-loxP / Wnt signaling / GSK-3β / hair cells / 神経幹細胞 / 器官培養 / 細胞移植 |
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| Research Abstract |
1.Knockout of RBP-J
Notch signaling is the important signal transduction pathway for cell proliferation and differentiation. Inhibition of this system enables the unusual cell proliferation that lead to the regeneration of hair cell. Using adenovirus vector and Cre-loxP system in the culture preparation of organ of Corti, we knocked out the gene of RBP-J : a transcription factor of Notch signaling. We achieved the ectopic generation of hair cells.
2.Pharmacological inhibition of RBP-J
In the culture preparation of organ of Corti, RBP-J was blocked by γ-secretase. Similarly to the genetic inhibition of RBP-J, ectopic hair cells were generated.
3.Knockout of GSK-3β
Wnt signaling is the important signal transduction pathway for cell proliferation. Activating Wnt signaling enable abnormal cell proliferation. We knocked out GSK-3β ; a inhibiting factor of Wnt signaling with the similar method to the RBP-J. We found the ectpic hair cell growth.
4.Pharmacological inhibition of GSK-3β
In the culture preparation of organ of Corti, GSK-3β was blocked by several drugs. Similarly to the genetic inhibition of RBP-J, ectopic hair cells were generated.
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