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Establishment of Experimental Autoimmune Inner Ear Disease Model Using New Immunological Procedures

Research Project

Project/Area Number 15591821
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionOita University (2004-2005)
大分医科大学 (2003)

Principal Investigator

SUZUKI Masashi (2004-2005)  Oita University, Faculty of Medicine, Department of Otolaryngology, Professor, 医学部, 教授 (60211314)

一宮 一成 (2003)  大分大学, 医学部, 助教授 (70223112)

Co-Investigator(Kenkyū-buntansha) YOSHIDA Kazuhide  Oita University, Faculty of Medicine, Department of Otolaryngology, Assistant, 医学部, 講師 (10305055)
鈴木 正志  大分大学, 医学部, 教授 (60211314)
Project Period (FY) 2003 – 2005
Project Status Completed (Fiscal Year 2005)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2005: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2004: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2003: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordstype II collagen / CpG motif / superantigen / MRL / lpr mouse / Freund's complete adjuvant / IgG / ICAM-1 / Stria vascularis / マウス / 聴性脳幹反応 / Mycobacterium tuberculosis / フロインドの完全アジュバンド / 関節炎 / アジュバント / lpr / 蝸牛 / 自己免疫病 / 難聴 / コラーゲン / CpG
Research Abstract

Mice were sensitized with (1)type II collagen, (2)CpG motif and type II collagen, or (3)Staphylococcal enterotoxin B (superantigen) and type II collagen, and examined hearing level by auditory brainstem responses. No obvious hearing loss was detected in each group. Sensitization of type II collagen might not be strong enough in our experiments because arthritis seen in each group was slight.
Hearing level of MRL/MpJ-lpr/lpr (MRL/lpr) mice was examined after they were injected with (4)superantigen or (5)Freun' s complete adjuvant (FCA). Some MRL/lpr mice showed impaired hearing after the sensitization with FCA.
Hematoxylin and eosin staining could not reveal obvious pathologic changes in the cochleas of the MRL/lpr mice with hearing loss. Immunostaining for IgG and ICAM 1 was observed on the endothelia of the stria vascularis vessels of the MRL/lpr mice. These findings, however, were seen not only in the mice with hearing disturbance after FCA injection, but also in the animals without hearing loss after the injection with FCA or saline. Further studies of the immune responses on the stria vascularis vessels might be important to understand the pathophysiology of the autoimmune inner ear diseases.

Report

(4 results)
  • 2005 Annual Research Report   Final Research Report Summary
  • 2004 Annual Research Report
  • 2003 Annual Research Report

Research Products

(11 results)

All 2005 2004 Other

All Journal Article (8 results) Publications (3 results)

  • [Journal Article] Dexamethasone inhibits tumor necrosis factor- a - induced cytokine secretion from spiral ligament fibrocytes2005

    • Author(s)
      Maeda Ke., Yoshida K., Ichimiya I., Suzuki M.
    • Journal Title

      Hearing Research 202

      Pages: 154-160

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Immunohistochemical detection of cervical lymph node micrometastases from T2NO tongue cancer2005

    • Author(s)
      Yoshida K., Kashima K., Suenaga S., Nomi N., Shuto J., Suzuki M.
    • Journal Title

      Acta Oto-Laryngologica 125

      Pages: 654-658

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Dexamethasone inhibits tumor necrosis factor-α- induced cytokine secretion from spiral ligament fibrocytes2005

    • Author(s)
      Maeda Ke., Yoshida K., Ichimiya I., Suzuki M.
    • Journal Title

      Hearing Research 202

      Pages: 154-160

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Immunohistochemical detection of cervical lymph node micrometastases from T2N0 tongue cancer2005

    • Author(s)
      Yoshida K., Kashima K., Suenaga S., Nomi N., Shuto J., Suzuki M.
    • Journal Title

      Acta Oto-Laryngologica 125

      Pages: 654-658

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2005 Final Research Report Summary
  • [Journal Article] Dexamethasone inhibits tumor necrosis factor-α-induced cytokine secretion from spiral ligament fibrocytes2005

    • Author(s)
      Maeda Ke., Yoshida K., Ichimiya I., Suzuki M.
    • Journal Title

      Hearing Research 202

      Pages: 154-160

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Immunohistochemical detection of cervical lymph node micrometastases from T2N0 tongue cancer2005

    • Author(s)
      Yoshida K., Kashima K., Suenaga S., Nomi N., Shuto J., Suzuki M.
    • Journal Title

      Acta Oto-Larygologica 125

      Pages: 654-658

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Dexamethasone inhibits tumor necrosis factor-alfa-induced cytokine secretion from spiral ligament fibrocytes2005

    • Author(s)
      Maeda K., Yoshida K., Ichimiya I., Suzuki M.
    • Journal Title

      Hearing Research (in press)

    • Related Report
      2004 Annual Research Report
  • [Journal Article] 蝸牛の免疫反応と難聴病態2004

    • Author(s)
      一宮一成, 吉田和秀, 鈴木正志
    • Journal Title

      第4回鎌倉カンファレンス記録集

      Pages: 72-84

    • Related Report
      2004 Annual Research Report
  • [Publications] 一宮一成: "中耳炎の蝸牛におよぼす影響"耳鼻免疫アレルギー. 21(4). 17-22 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] Ichimiya, I., et al.: "Expression of adhesion molecules by cultured spiral ligament Fibrocytes stimulated with proinflammatory cytokines"Ann Otol Rhinol Laryngol. 112(8). 722-728 (2003)

    • Related Report
      2003 Annual Research Report
  • [Publications] 一宮一成: "ラセン靱帯の関わる難聴病態"Otol Jpn. 13(2). 96-99 (2003)

    • Related Report
      2003 Annual Research Report

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Published: 2003-03-31   Modified: 2016-04-21  

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