| Project/Area Number |
15591849
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Ophthalmology
|
|---|
| Research Institution | SHINSHU UNIVERSITY |
|---|
Principal Investigator |
KIKUCHI Takanobu SHINSHU UNIVERSITY, RESEARCH CENTER FOR HUMAN AND ENVIRONMENTAL SCIENCE, PROFESSOR, ヒト環境科学研究支援センター, 教授 (50177797)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OHTA Koichi SHINSHU UNIVERSITY, OPHTHALMOLOGY, ASISTANT PROFESSOR, 医学部附属病院, 講師 (70262730)
吉村 長久 信州大学, 医学部, 教授 (70211662)
|
|---|
| Project Period (FY) |
2003 – 2004
|
| Project Status |
Completed (Fiscal Year 2004)
|
| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2003: ¥2,000,000 (Direct Cost: ¥2,000,000)
|
|---|
| Keywords | Cancer-associated retinopathy / autoantibody / retinal antigen / PTB-like protein / RNA binding proteins / RNA結合蛋白 |
|---|
| Research Abstract |
Neuronal RNA-binding proteins play an important role in post-transcriptional regulation of gene expression such as alternative splicing, RNA transport and local translation. Several families of neuronal RNA-binding proteins have been so far described. For example, Hu proteins have been identified as an autoantigen of human paraneoplastic encephaloneuropathies associated with lung small cell carcinomas. One of the major functions of Hu proteins is regulation of mRNA metabolism through binding to RNA stability elements. Nova-1, known as the paraneoplastic opsoclonus-myoclonus ataxia antigen, is also a neuron-specific RNA binding protein that regulates alternative splicing. Recently, we have cloned a homologue of PTB as a possible autoantigen of cancer-associated retinopathy from human and rat cDNA libraries. This new protein was named as PTB-like protein (PTBLP).
In this study, the possible roles of PTBLP on neuronal differentiation were examined using PC12 cells and PTBLP null mice. Duri
… More
ng NGF-induced neuronal differentiation in PC12 cells, PTBLP-L (long form) was down-regulated. By transfection of PTBLP-L into PC12 cells, the neuronal differentiation was suppressed. In PTBLP-S (short form ; lack the RNA binding ability) transfected cells, however, this suppression was not evident. When both PTBLP-L and PTBLP-S were co-transfected, the suppressive effect of PTBLP-L decreased. In differentiated cells, PTBLP-S localized in the nucleus and PTBLP-L was found dispersed throughout the cytoplasm and neuronal growth cone. These findings suggest that PTBLP-L acts as a negative regulator of neuronal differentiation and PTBLP-S acts as a competitor of PTBLP-L. To explore the functions of PTBLP in the intact animal, we undertook to knock out the PTBLP gene in mice by homologous recombination. PTBLP null mice were died in embryonic late stage. There was no abnormal phenotype in PTBLP heterozygotes. Those results are suggested that PTBLP may play an important role in neuronal development. Less
|
