| Project/Area Number |
15591876
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | KITASATO UNIVERSITY |
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Principal Investigator |
TAKANO Masahiko Kitasato University, School of Medicine, Assistant Professor, 医学部, 講師 (40236253)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2004: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 2003: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | retinal ganglion cell / axonal regeneration / neural cell death / retrograde labeling / gene transfer / neurotrophic factor / optic nerve graft / fluorescent dye |
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| Research Abstract |
We used a couple of neural survival factor, BDNF(brain-derived neurotrophic factor) and GDNF(glial cell-line derived neurotrophic factor) provided by investigation of the culture system of adult mammalian retina in last year, and confirm their effects by in vivo analysis. Retinal ganglion cells(RGCs) of adult rat were pre-labeled by retrograde labeling of fluorescent dye(DiI). Then GDNF gene was transfer into RGCs. After the intra-vitreous injection of plasmid gene, five electric pulses were delivered twice with 5 min was transfer into RGCs using contact lens-type electrode. Then the left optic nerve was completely cut intra-orbitally under an anesthesia. Two and 4 weeks after the operation, the retina was isolated after an extraction of eyeball, and the labeled RGCs were observed and measured the number of the living cells under a fluorescent microscope. After the electro-induction, a significant increase in the number of surviving cells. As a result what both factor acted on as a preventive factor and a neurotrophic factor on neuronal cell death of an adult retina in vivo, same as results from the retinal culture system. Therefore both BDNF and GDNF are thought to be useful in functional reconstruction of a visual system in adult mammals.
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