| Project/Area Number |
15591885
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatric surgery
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| Research Institution | Niigata University |
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Principal Investigator |
YAMAZAKI Sataru Niigata University, Medical and Dental Hospital, assistant, 医歯学総合病院, 助手 (20334683)
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| Co-Investigator(Kenkyū-buntansha) |
KUBOTA Masayuki Niigata University, Graduate School of Medical and Dental sciences, professor, 大学院・医歯学総合研究科, 教授 (50205150)
TANAKA Shinji Niigata University, Graduate School of Medical and Dental sciences, assistant, 大学院・医歯学総合研究科, 助手 (30361926)
金田 聡 新潟大学, 医歯学総合病院, 講師 (90313535)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2004: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2003: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | β-catenin / immunostaining / hepatoblastoma / Wilms' tumor / neuroblastoma / 定量的PCR |
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| Research Abstract |
β-catenin expression were studied in 54 solid tumors of children consisting of 29 neuroblastomas, 10 hepatoblastomas, 12 Wilms' tumor, one colon cancer, and one cholangiocarcinoma. Surgical resection specimens were obtained at Niigata University Medical and Dental Hospital since 1982 to 2004. Paraffin embedded blocks of each tumor tissue were sliced to 5 μm section, and were immunohistochemically stained for β-catenin with SAB method. Localization of β-catenin protein expression was investigated using a monoclonal mouse anti-β-catenin antibody. Manifestation in nucleus of tumor cells were detected as expression abnormalities of β-catenin. In neuroblastoma, colon cancer, and cholangiocarcinoma, abnormal accumulation was detected in neither nucleus nor cytoplasm. In hepatoblastoma cases, both nucleus and cytoplasm were dyed in 60% (6/10) cases, and only cytoplasm was stained in 2 cases. As for Wilms' tumor, cytoplasm was dyed in 15% (3/12) cases, and nucleus was not stained. Significant abnormalities of β-catenin expression were detected only in hepatoblastoma cases. Farther investigation is required for suitable evaluation on colon cancer and cholangiocarcinoma.
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