| Project/Area Number |
15591888
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Pediatric surgery
|
|---|
| Research Institution | KYUSHU UNIVERSITY |
|---|
Principal Investigator |
IEIRI Satoshi KYUSHU UNIVERSITY, University Hospital, Research Associate, 大学病院, 助手 (00363359)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SUITA Sachiyo KYUSHU UNIVERSITY, University Hospital, Mayor of Hospital, 大学病院, 病院長 (30038856)
TAGUCHI Tomoaki KYUSHU UNIVERSITY, Faculty of Medicine, Professor, 大学大学院, 教授 (20197247)
TAJIRI Tatsurou KYUSHU UNIVERSITY, Faculty of Medicine, Associate Professor, 大学大学院, 助教授 (80304806)
OGITA Keiko KYUSHU UNIVERSITY, Faculty of Medicine, Research Associate, 大学大学院, 助手 (40346771)
UESUGI Toru KYUSHU UNIVERSITY, University Hospital, The Medical Staff, 大学病院, 医員 (30380421)
|
|---|
| Project Period (FY) |
2003 – 2004
|
| Project Status |
Completed (Fiscal Year 2004)
|
| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2004: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2003: ¥1,500,000 (Direct Cost: ¥1,500,000)
|
|---|
| Keywords | Persistent pulmonary hypertension of newborn / Vascular smooth muscle / Vasodilator / Pulmonary artery / Intracellular Ca concentration / Mechanical ventilation / Cytokine / Stress / 細胞内カルシウム濃度 |
|---|
| Research Abstract |
The aim of this study is to elucidate the effect of stress (mechanical ventilation) in neonatal vascular smooth muscle using a rat model. Pregnant Sprague-Dawley rats were treated by experimental protocols on day 21 of gestation. We used the following two groups : The fetal stabilization group (FS-group) which consisted of neonatal rats treated by fetal stabilization. They received morphine hydrochloride (3mg/kg) via the placenta by means of maternal intra-abdominal injection before undergoing a caesarean section. In the control group (C-group), they received no morphine hydrochloride during the perinatal period. Tracheal intubations were performed in neonates after caesarean section in both groups. All neonatal rats were managed under mechanical ventilation for 4 hours. We collected the samples at birth and at 4 hours after birth in both groups and then measured the serum Tumor-necrosis-factor-alpha (TNF-α), Interleukin-6 (IL-6) concentrations and the TNF-α concentration in BALF by ELISA. The specimens obtained from the right lung were immunohistochemically stained with anti-mouse TNF-α polyclonal antibody. The levels of serum TNF-α at birth and IL-6 at 4 hours after birth in the C-group increased, in comparison to that in the FS-group. At birth in the C-group, the TNF-α concentration in BALF tended to be higher than that in the FS-group. TNF-α positive cells were identified in the bronchial muscular layers, arterial media and externa in both groups. The staining intensity in the FS-group was weaker than that in the C-group both at birth and at 4 hours after birth. Our result suggests that the regulation of the production of inflammatory cytokines may regulate the action of vascular smooth muscle.
|
