| Project/Area Number |
15591927
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Niigata University |
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Principal Investigator |
KAWANO Yoshiro Niigata University, Graduate School of Medical and Dental Sciences, Assistant, 大学院・医歯学総合研究科, 助手 (60303129)
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| Co-Investigator(Kenkyū-buntansha) |
MAEDA Takeyasu Niigata University, Graduate School of Medical and Dental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (40183941)
AMIZUKA Norio Niigata University, Graduate School of Medical and Dental Sciences, Associate Professor, 大学院・医歯学総合研究科, 助教授 (30242431)
NOZAWA Kayoko (INOUE Kayoko) Niigata University, Graduate School of Medical and Dental Sciences, Assistant, 大学院・医歯学総合研究科, 助手 (90303130)
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| Project Period (FY) |
2003 – 2004
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| Project Status |
Completed (Fiscal Year 2004)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2004: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2003: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Shh / development / immunohistochemistry / ENaC / HSP90 / incisor / RT-PCR / morphology / 器官培養 / エナメル芽細胞 / 中間層細胞 / 分化 / shh / 微細構造学 |
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| Research Abstract |
The amiloide-sensitive epithelial sodium channel (ENaC) consisting of three subunits (α,β,γ) is an apical membrane protein which involves control of cell volume and mechano-transduction. These subunits are co-expressed in renal collecting duct and distal colon epithelium at equivalent levels. We have investigated their expression at mRNA level in the dental epithelium of the rat incisor, based on the differentiation stages. RT-PCR analysis demonstrated clear bands for three subunits mRNA in the samples obtained from the enamel organs of early and late maturation stages and secretory stage. Furthermore, in situ hybridization histochemistry and immunocytochemistry confirmed the region-specific expression of Alpha-and Gamma-ENac in the ameloblasts layer.
Heat Schock proteins are expressed as chaperons by some cells living in physiological as well as stressful conditions. The Hsp90 functions in the pathways of numerous signal transducers, cell cycle and developmental regulators under normal growth conditions. Our immunocytochemical examinations showed its expression throughout the dental epithelium during the amelobalst-differentiation. Interestingly, intense exhibition for Hsp90-immunoreaction was found in the cytoplasm of cementoblasts on the newly-formed dentin close to the epithelial root sheath.
Sonic hedgehog(Shh), a member of the mammalian hedge hog family, plays a key role during embryogenesis and organogenesis. Shh influences behavior and fate of cells located within its range of diffusion. We immunocytochemically investigated the distribution of Shh protein in the rat incisor. Abundant immunoreation for Shh was recognizable both in multi-layered stratum intermedium and its adjacent inner dental epithelium. Immunoreation became weak in the ameloblasts towards cervical regions. Furthermore, Shh-immunoreation was distributed in the mesenchyme close to inner dental epithelium in a gradient manner.
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